Kodi Kuyesa Kwapafupi Kwambiri Kuyesa ndi Chiyani?

Kutuluka kotsatira kuyesa (LFA) ndi njira yodziwika bwino yoyezetsa poyeserera (POC) ndipo chifukwa chake ndi yotsika mtengo komanso yosavuta kugwiritsa ntchito. Kufufuza Kwotsatira tests are used to identify biomarkers in any sample of urine, saliva, blood, plasma, etc. for diagnostic purposes. Conventional LFAs produce semi-quantitative or qualitative results, necessitating the use of specialized instruments to detect quantitative results. LFA is a paper-based testing method for detecting and quantifying analytes in complex mixtures. The sample is placed on a test device, and the results are presented within five to thirty minutes, approximately. LFAs’ applications have broadened to a number of areas requiring rapid testing due to their cost-effectiveness and ease of manufacture.

Makhalidwe apamwamba a mayeso ofananira oyeserera akufotokozedwa motsatira. Choyamba, ndi zotayika zomwe zikutanthauza kuti sizingagwiritsidwe ntchito mobwerezabwereza. Kuphatikiza apo, sizitsuka. Mukazigwiritsa ntchito ziyenera kutayidwa. Kachiwiri, ndizachuma ndipo motero zimapezeka mosavuta. Chachitatu, ndizolimba mwachilengedwe, zomwe zikutanthauza kuti zimatha kusungidwa kutentha ndikukhala ndi moyo wazaka zingapo.

M'zaka zaposachedwa, pakhala msika womwe ukukula ndikufunika kwamayeso angapo opatsirana pogwiritsa ntchito mizere yoyeserera yomwe imalola kuti ma analytiki angapo azitsatira mosamala mosamala. Kuyesa kotere (mwina imodzi LFA) ziyenera kukhala zosalala bwino popanda kuthandizidwa ndi labotale kapena magulu a akatswiri azamagetsi. Ma LFA ndi njira yosankhika chifukwa ndi yotsika mtengo kupanga, yosavuta kugwiritsa ntchito, ndipo koposa zonse, imadziwika ndi ogwiritsa ntchito kumapeto ndi oyang'anira.

Pofuna kutchula mitundu yoyeserera yoyenda yotsatira, pali mitundu iwiri yoyeserera koyeserera. Mitundu yoyeserera yoyeserera yoyeserera imachokera pachitsanzo choyesedwa. Mitundu ikuluikulu iwiri ili ndi magulu ena ang'onoang'ono. Komabe, mtundu woyamba wa mayendedwe otsekemera amatchedwa Lateral Flow Immuno Assay ndipo mtundu wachiwiri wa LFA umatchedwa Nucleic Acid Lateral Flow. Otsatira otaya immuno assay amagawika magawo awiri. Imodzi imagwira ntchito potengera ma antibodies omwe amakhala gawo lalikulu lazitsanzo kuti adziwe. Pomwe chachiwiri chimakhala ndi mawonekedwe ozindikira kapena zosakaniza monga mapuloteni, mahomoni, ndi zina. Mtundu wachiwiri wa Lateral Flow Assay umatchedwa Nucleic ACid lateral flow assay yomwe imathandizira kupeza ma amplicon omwe mwina adapangidwa panthawi polymerase unyolo anachita (PCR).

Kuti mupange mayeso a Lateral Flow Assay pali njira ziwiri zomwe zingagwiritsidwe ntchito. Njira yoyamba imatchedwa mtundu wa dipstick pomwe mtundu wachiwiri umatchedwa kaseti yosungidwa. Ntchito zawo ndizofanana komabe zimadalira mtundu, zofunikira pamsika, matrix, ndi makampani. Pakuyesa kwa lateral Assay Test, pali mitundu iwiri yomwe imagwira ntchito. Imodzi imadziwika kuti sangweji ndipo yachiwiri imadziwika ndi dzina la mpikisano. Mtundu wa sangweji umagwiritsidwa ntchito pozindikira ma analytics akulu omwe angakhale ndi masamba awiri omangiriza kapena ma epitopes. Zotsatira zamtundu wa sangweji zimakhala zowoneka bwino pamzere woyeserera womwe umawongolera molingana ndi kuchuluka kwa wofufuza yemwe ali mchitsanzocho. Kuchotsa zomwe zimachitika pakuwunika, ngati chingwe chowongolera chili ndi mitundu yolimbana ndi ma antibody chidzakhala ndi mphamvu zomanga ma nanoparticles omwe apitilizabe kutulutsa chizindikiritso cha mzere wowongolera kuti awonetse magwiridwe antchito a lateral flow assay.

M'mayeso a Lateral flow assay, ma nanoparticles amakhala atolankhani chifukwa cha mayeso ena. Zotsatira za mayeso zitha kuwerengedwa ndikuwunikiridwa kudzera m'maso omwe amatchedwanso oyenerera kapena owerengeka kapena mothandizidwa ndi chida chomwe chimatchedwa kuwerengera kochulukirapo. Kuyesa kwamayeso ofananira ndi kotheka kumagwira ntchito ndipo kumatha kubweretsa zotsatira zowona komanso zofunika kwambiri kukula kwa ma particles pakati pa 20 nm mpaka 50 nm. Kuti chizindikirocho chikhale cholimba, pali tinthu tating'onoting'ono tomwe titha kugwiritsidwa ntchito ngati chisonyezo champhamvu kwambiri pakupanga kapena zotsatira za chitsanzocho chifukwa chake. Potengera mtundu wopikisana wa kuyesa kwa Lateral flow Assay, amagwiritsidwa ntchito pozindikira ma analytics makamaka ngati awiriawiri a antibody sapezeka kapena wowerengera si wamkulu kwenikweni chifukwa cha zochitika zambiri zomanga ma antibody. Mzere woyeserera nthawi zambiri umakhala ndi molekyulu ya analyte, yomwe nthawi zambiri imakhala yosakanikirana ndi mapuloteni, ndipo phukusi la conjugate limapangidwa ndi anti-nanoparticle conjugate mu mpikisano wapa Lateral Flow Assay Test. Ngati wowunikira akuwonekeratu, a conjugate adzamangiriza, kuletsa kuti asamangomangika kwa wowunikira pamzere woyesera.

Kusanthula kwamayeso oyesa ofananira nawo kutengera mitundu itatu yazotsatira zomwe zingakhale zosokoneza. Komabe, mitundu itatu yayikulu ya zotsatirazi ndiyabwino, yochulukirapo, kapena yochulukirapo. Pakuwunika kwakanthawi kwamayeso oyesa ofananira nawo, mzere woyeserera umafanizidwa ndi muyezo womwe umadziwika kuti calibration standard, kenako umasandulika kukhala mtengo wowerengera wa analyte. Komabe, kuti mumve zolondola komanso pazotsatira zake, owerenga mzere amalimbikitsidwa kuti azigwiritsa ntchito kupenda ndi kuwerengera mayeso.

Kutsiliza

Kuyesa Kwamafufuzidwe Otsatira ndi njira imodzi yomwe imagwiritsidwa ntchito kwambiri komanso yodziwika padziko lonse lapansi yomwe ili ndi zida zapadera. Kuphatikiza apo, Kuyesa Kwotsatira Kwawo kwakhala gawo lothandizira kwambiri pantchito zazaumoyo komanso m'maiko omwe akutukuka kumene ntchito yawo ndi yayikulu. Kugwiritsa ntchito kwawo ndiubwino wawo sikumangokhala pama labotale okha; M'malo mwake akugwiritsidwa ntchito poyesera kunyumba ndipo atsimikizira kuti ndi othandiza, osavuta kugwiritsa ntchito, komanso osafuna ndalama zambiri. Chifukwa chake, kuyesa kwamayeso amtundu woyeserera ndi mitundu yawo yosiyanasiyana kumathandizira pazidziwitso zosiyanasiyana ndipo zipitilira kukula ndi zida zina zikaphatikizidwa kuti zibweretse kupita patsogolo ndikukula m'chigawo chino.

Zothandizira
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986465/
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics#target
https://www.clinisciences.com/en/read/serological-tests-in-mycology-1190/lateral-flow-assay-lfa-2095.html