Asanayambe kugwira ntchito ndi mfundo za Otsatira Otsatira Assay ndikofunikira kufotokoza mwachidule za Kufufuza Kwotsatira. Kuyenda Kwotsatira kapena kuyitanidwa ndi dzina lina mwachitsanzo immunochromatography imagwiritsidwa ntchito kuzindikira kupezeka kapena kupezeka kwa ma analytics kuti mupeze zomwe mukufuna. Omwe akutenga nawo mbali mgululi ndi antigen, mitundu yama antibody, ndi fanizo kapena matrix. Komanso, Kufufuza Kwotsatira amagwiritsidwa ntchito pozindikira kapena kuzindikira monga amagwiritsidwira ntchito pozindikira khansa ndi chikhansa, kutenga pakati, kuzindikira kugwiritsa ntchito mankhwala osokoneza bongo kapena bongo, tizilombo toyambitsa matenda, matenda opatsirana, komanso zolembera zamtima.
Ntchito Yotsatira ya Flow Assay idakhazikitsidwa ndi magawo anayi. Choyambirira, ndichida chomwe chili ndi pad, yoyeserera, mzere woyeserera, ndi chingwe chowongolera (nitrocellulose Mzere). Gawo lirilonse la izi limasiyanitsidwa ndi kamtunda kakang'ono kamene kamalola kuyeserera koyeserera. Gawo loyamba logwiritsira ntchito chipangizochi ndikuti chitsanzocho chimayikidwa pachida chomwe chimayendera kutsata kotsalira kwathunthu. Ikangoyenda, kusefako kumachitika kumene magawo osafunikira kapena osafunikira amachotsedwa kapena kusefedwa. Mkati mwa nthawi yomwe mwayesedwa kuyesa kumaliza, kuyesa konse kumachitika motero zotsatira zimawoneka. Chitsanzocho chikamafika panjira yolumikizira ya chipangizocho, ma particles owumawo amakhala osakanikirana ndi tinthu ting'onoting'ono.
Gawo lotsatira lomwe limachitika ndikutuluka kwa ma nanoparticles omangika ndi ma analyte ndikupeza mwayi wolumikizana ndi mizere yomwe imabweretsa zotsatira. Choyikiracho chimatha kuyamba kuyenderera kupaketi ya conjugate, yomwe imakhala ndi ma nanoparticles ofiira kwambiri kapena owala kwambiri okhala ndi anti antibody pamwamba pake. Palinso chida cholowerera chomwe chimayamwa sampuli kapena choledzeretsa kwathunthu kuti zitsimikizire kuti palibe madzi otsalira mozungulira mayesowo ndi mizere yolamulira. Mzere wolamulira wazitsulo zoyenda mozungulira womwe umatchedwa C umapangidwa ndi ma gland oyandikana omwe amathandizanso kulumikiza nanoparticle conjugate kapena popanda kukhalapo kwa wofufuza mu yankho kuti awonetsetse kuti zofunazo zikugwira ntchito moyenera osati mwanjira ina . Kwenikweni, njira yoyesera pogwiritsa ntchito chipangizochi idakhazikitsidwa ndi zomwe zimayambitsa antigen-antibody reaction. Monga tafotokozera pamwambapa mayeso oyeserera ofananira nawo amatha kupangitsa kuwerengera m'njira ziwiri kapena kuwunika kwakanthawi.
Kuwitsogolera kufotokozera momveka bwino, gawo loyamba ndi lachitsanzo pomwe padutsamo nyembazo kenako ndikulowetsa. Pali mwayi kuti fyuluta itha kukhala papepala lachitsanzo kuti izitsata zolondola komanso zofunikira pachitsanzo. Gawo lachiwiri lili pa phukusi la conjugate. Pedi ya conjugate imayamba kugwira ntchito pambuyo poti nyerereyo yatengeka ndipo ili ndi zilembo ndi ma antibodies omwe adzawerengera ngati ma analytiki alipo pamayesowo kapena ayi pazochitika zomangiriza. Pambuyo pa magawo awiriwa, kugwira ntchito kwa nembanemba ya nitrocellulose kumayamba kugwira ntchito. Pa nembanemba ya nitrocellulose, ma analytic kapena ma antibodies omwe amapezeka muzitsanzo ndi conjugated pad adzalumikizana ndi a nitrocellulose pad. Pambuyo pake, ipitilira pad yozolowereka yomwe ingakuthandizeni kupeza zina zowonjezera ndikupanga zotsatira.
Kupereka chitsanzo cha ntchito ya Mayeso Omwe Akuyenda Kuyesa, Chitsanzo cha katemera woyeserera wa antibody wofulumira kwambiri wa Covid-19 chingatengedwe. Chitsanzocho kapena chojambulacho chikuwonjezeredwa pachitsanzo cha pulogalamu yoyeserera yoyeserera imadutsanso kanema yemwe ali pachida chotchedwa fyuluta. Ikadutsa kupezeka kapena kupezeka kwa omwe akuwunikira adzayesedwa ndipo ngati chitsanzocho chidzakhala ndi ma analytics ofunikira chidzaphatikizana ndi antigen yomwe imatchedwa colloidal-gold label antigen yomwe idzakhale ndi gulu lachikuda. Ngati pali gulu lachikuda limatanthawuza kupezeka kwa ma analytics omwe akuwonekera mu specimen yomwe idawonjezeredwa pakuyesa kwa Lateral flow assay kuyesa ndi kuyesa. Ndipo ngati mulibe mzere wachikuda zikutanthauza kuti zotsatira zake ndizosavomerezeka Pomaliza, pali mzere wowongolera wazida za Lateral flow assay zomwe zikuwonetsa kuti zitsanzozo zawonjezedwa pachidacho.
Kuti mumvetsetse momwe kuyeserera kotsatira kumagwirira ntchito ndikofunikanso kukambirana kumasulira kwa zotsatira. Choyamba, ndikofunikira kumvetsetsa magawo osiyanasiyana a chipangizocho monga conjugate pad, chitsanzo pad, ndi zina zotero, ma antibodies olumikizana atakumana pamizere yolamulira, mizere imayamba kuwoneka kuti ikuwonetsa zotsatira. Mizere yooneka pazolemba zagolide za nanosphere zomwe ndizolemba zomwe zimagwiritsidwa ntchito kwambiri, ndizofiira ndipo sizifuna chitukuko chilichonse. Makulidwe a nanoparticles wagolide dziwani mitundumitundu yofiira, ndipo mphamvu imawonetsedwa ndikulowera kwa ma anti-conjugated omwe atsekedwa pamizere yopanda mphamvu. Ngati mzere wofiira ukuwonekera pamzere woyeserera ndiye kuti zikuwonetsa kuti zotsatira zake ndi zabwino. Kulankhula za kulimba kwake, zimatengera kuchuluka kwa wopendekera yemwe ali pamasewerawa. Komanso, mawonekedwe olamulira pazida zoyeserera zoyendetsera mosalekeza ndiyofunika kuti awonetsetse kuti zitsanzo zokwanira zaperekedwa ndipo ndalamazo sizocheperako poyesa ndikupereka zotsatira zolondola.
Kugwira ntchito kwa Kufufuza Kwotsatira ndizosavuta komanso zomveka. Popeza samangokhala o ma laboratories m'malo mwake amangogwirira ntchito m'malo a labotale momwemo munthu amatha kuwagwiritsa ntchito ndikumvetsetsa magawo osiyanasiyana. Kutulutsa kodabwitsa kwa chida chotsatira chotsatira, ndi nanoparticles wagolide, apangitsa kuyezetsa kukhala njira yosavuta komanso yofikirika. Ali ndi gawo lalikulu pantchito zachipatala komanso matenda.
Zothandizira
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test
https://www.abingdonhealth.com/services/what-is-lateral-flow-immunoassay/
https://www.abingdonhealth.com/services/what-is-lateral-flow-immunoassay/
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics#target
