Kodi Kuyesa Kwotsatira Kumagwira Ntchito Motani?
Musanalowe mu mfundo zogwirira ntchito ndi zogwirira ntchito za Lateral Flow Assay, ndikofunikira kupereka mawu oyamba mwachidule okhudza Lateral Flow Assay . Lateral Flow Assay kapena kutchedwa ndi dzina lina mwachitsanzo immunochromatography imagwiritsidwa ntchito kuzindikira kupezeka kapena kusakhalapo kwa analytes kuti mupeze zotsatira zomwe mukufuna. Ogwira ntchito akuluakulu omwe ali mgululi ndi antigen, mitundu ya ma antibody, ndi chitsanzo kapena matrix. Komanso, Lateral Flow Assays imagwiritsidwa ntchito pazinthu zingapo zozindikira kapena zodziwira matenda monga momwe zimagwiritsidwira ntchito kuzindikira khansa pogwiritsa ntchito chizindikiro cha khansa, mimba, kuzindikira kugwiritsa ntchito mankhwala osokoneza bongo kapena kumwa mopitirira muyeso, tizilombo toyambitsa matenda, matenda opatsirana, komanso zizindikiro za mtima.
Kugwira ntchito kwa Lateral Flow Assay kumadalira mbali zinayi. Choyamba, ndi chipangizo chomwe chili ndi chitsanzo cha pad, conjugate pad, mzere woyesera, ndi mzere wowongolera ( nitrocellulose strip ). Gawo lililonse la izi limalekanitsidwa ndi mtunda waung'ono womwe umalola kuyenda bwino kwa chitsanzocho. Gawo loyamba logwiritsa ntchito chipangizochi ndikuti chitsanzocho chiyikidwe pa chipangizo chomwe chimayendetsedwa ku chipangizo cha lateral flow mokwanira. Chikayenda, njira yosefera imachitika pomwe tinthu tosafunikira kapena tosafunikira timachotsedwa kapena kusefedwa. Mkati mwa nthawi yoperekedwa yomaliza mayeso a Lateral flow assay, njira yonse imachitika ndipo zotsatira zake zitha kuwoneka. Chitsanzocho chikafika pa conjugate pad ya chipangizocho, tinthu touma pa pad timasakanikirana ndi tinthu ta chitsanzocho.
Gawo lotsatira lomwe limachitika ndikutuluka kwa ma nanoparticles omangika ndi ma analyte ndikupeza mwayi wolumikizana ndi mizere yomwe imabweretsa zotsatira. Choyikiracho chimatha kuyamba kuyenderera kupaketi ya conjugate, yomwe imakhala ndi ma nanoparticles ofiira kwambiri kapena owala kwambiri okhala ndi anti antibody pamwamba pake. Palinso chida cholowerera chomwe chimayamwa sampuli kapena choledzeretsa kwathunthu kuti zitsimikizire kuti palibe madzi otsalira mozungulira mayesowo ndi mizere yolamulira. Mzere wolamulira wazitsulo zoyenda mozungulira womwe umatchedwa C umapangidwa ndi ma gland oyandikana omwe amathandizanso kulumikiza nanoparticle conjugate kapena popanda kukhalapo kwa wofufuza mu yankho kuti awonetsetse kuti zofunazo zikugwira ntchito moyenera osati mwanjira ina . Kwenikweni, njira yoyesera pogwiritsa ntchito chipangizochi idakhazikitsidwa ndi zomwe zimayambitsa antigen-antibody reaction. Monga tafotokozera pamwambapa mayeso oyeserera ofananira nawo amatha kupangitsa kuwerengera m'njira ziwiri kapena kuwunika kwakanthawi.
Kuwitsogolera kufotokozera momveka bwino, gawo loyamba ndi lachitsanzo pomwe padutsamo nyembazo kenako ndikulowetsa. Pali mwayi kuti fyuluta itha kukhala papepala lachitsanzo kuti izitsata zolondola komanso zofunikira pachitsanzo. Gawo lachiwiri lili pa phukusi la conjugate. Pedi ya conjugate imayamba kugwira ntchito pambuyo poti nyerereyo yatengeka ndipo ili ndi zilembo ndi ma antibodies omwe adzawerengera ngati ma analytiki alipo pamayesowo kapena ayi pazochitika zomangiriza. Pambuyo pa magawo awiriwa, kugwira ntchito kwa nembanemba ya nitrocellulose kumayamba kugwira ntchito. Pa nembanemba ya nitrocellulose, ma analytic kapena ma antibodies omwe amapezeka muzitsanzo ndi conjugated pad adzalumikizana ndi a nitrocellulose pad. Pambuyo pake, ipitilira pad yozolowereka yomwe ingakuthandizeni kupeza zina zowonjezera ndikupanga zotsatira.
Kuti tisonyeze momwe mayeso a Lateral Flow Assay amagwirira ntchito , chitsanzo cha zida zoyesera za Covid-19 zitha kutengedwa. Chitsanzocho chikawonjezedwa ku chitsanzo cha chipangizo choyesera cha lateral flow, chimadutsa mu filimu yomwe ili pa chipangizocho chotchedwa filter film. Chikaperekedwa, kupezeka kapena kusakhalapo kwa analyte yowunikira kudzayesedwa ndipo ngati chitsanzocho chidzakhala ndi analyte ofunikira chidzagwirizana ndi antigen yotchedwa colloidal-gold label antigen yomwe idzakhala ndi bande lamtundu. Ngati pali bande lamtundu, izi zimasonyeza kukhalapo kwa analyte yowunikira mu chitsanzo chomwe chinawonjezedwa ku mayeso a Lateral flow assay kuti chiyesedwe ndi kuzindikiritsa matenda. Ndipo ngati palibe mzere wamtundu, zikutanthauza kuti zotsatira zake sizolondola. Chomaliza koma chofunika kwambiri, pali mzere wowongolera wa chipangizo choyesera cha Lateral flow chomwe chikuwonetsa kuti kuchuluka kokwanira kwa chitsanzocho kwawonjezedwa ku chipangizocho.
Kuti timvetse momwe mayeso oyendera mbali amagwirira ntchito, ndikofunikiranso kukambirana tanthauzo la zotsatira zake. Choyamba, ndikofunikira kumvetsetsa magawo osiyanasiyana a chipangizocho monga conjugate pad , sample pad , ndi zina zotero. Chifukwa chake, ma antibodies olumikizidwa akasonkhana pamizere yowongolera, mizere imayamba kuwoneka kuti ikuwonetsa zotsatira zake. Mizere yowoneka pa malembo a nanosphere agolide omwe ndi malembo omwe amagwiritsidwa ntchito kwambiri, ndi ofiira ndipo safuna kupangidwa kulikonse. Miyeso ya tinthu tating'onoting'ono tagolide imatsimikizira mitundu yosiyanasiyana ya yofiira, ndipo mphamvu imawonetsedwa ndi malire a ma antibodies olumikizidwa omwe ali pamizere yosayenda. Ngati mzere wofiira uwonekera pamzere woyesera ndiye kuti zotsatira zake ndi zabwino. Ponena za mphamvu, zimatengera kuchuluka kwa analyte komwe kulipo mu matrix ya chitsanzo. Komanso, mawonekedwe a mzere wowongolera pa chipangizo choyesera mbali ndi chofunikira kuti zitsimikizire kuti kuchuluka kokwanira kwa zitsanzo kwaperekedwa ndipo kuchuluka kwake sikucheperako poyesa ndikupereka zotsatira zolondola.
Kutsiliza
Kugwira ntchito kwa Lateral Flow Assay n'kosavuta komanso komveka bwino. Popeza sikuti ndi ma laboratories okha, amagwira ntchito zina osati za labotale, munthu amatha kuzigwiritsa ntchito mosavuta ndikumvetsetsa magawo osiyanasiyana. Kapangidwe kabwino ka chipangizo choyesera cha lateral flow, ndi tinthu tating'onoting'ono tagolide , kwapangitsa kuti kuyesaku kukhale kosavuta komanso kosavuta. Kuli ndi gawo lalikulu pazifukwa zachipatala komanso zodziwira matenda.
Zothandizira
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test
https://www.abingdonhealth.com/services/what-is-lateral-flow-immunoassay/
https://www.abingdonhealth.com/services/what-is-lateral-flow-immunoassay/
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics#target