Othandizira Otsatira Pofufuza Zoyeserera Patsogolo

Kuyesa kwa mayendedwe a lateral ndi komwe kumayesedwa pogwiritsa ntchito zida zowunikira chisamaliro zomwe zili ndi zida zaukadaulo. Popeza zili ndi zida zaukadaulo, pali mwayi woti pakhale vuto lililonse kapena cholakwika chomwe chingasokoneze ogwiritsa ntchito. Kuti vutoli lithe, komanso kusanthula kwake, ndikofunikira kudziwa za njira yothetsera mavuto a Lateral Flow Assay . Kuyesa kwa lateral Flow Assay kungakhale ndi mavuto ena monga zotsatira zabodza, zotsatira zabodza zabodza, mavuto a nembanemba, ndi zina zambiri.

Buku lothandizira kuthetsa mavuto a kuyesa kwa lateral flow assay limakhala lofunikira kwambiri ngati pali chitsimikizo cha kulephera komwe kwanenedwa. Pa kulephera komwe kwanenedwa, munthu ayenera kufotokoza kulephera mu mayeso a lateral flow assay kuti pakhale chidziwitso chokwanira. Kuphatikiza apo, ndikofunikira kudziwa kuchuluka kwa kulephera, kuchuluka kwa nthawi zomwe zachitika, komanso kuchuluka kwa mwayi woti kulephera kumeneku kudzabwerenso.

Mu bukhu lothandizira kuthetsa mavuto, pali njira zingapo zomwe ziyenera kutsatiridwa ngati vuto litabuka mu mayeso oyendera mtunda ndi chipangizo. Gawo loyamba ndikufufuza zoyambira za LFA. Mu gawo ili, mbiri ya zinthu, ndi kusinthasintha kwa njira yoyesera ya LFA ziyenera kufufuzidwa. Komanso, ngati pali zinthu zachilendo zomwe zawonedwa poyesa, zidzakhalanso zothandiza pozindikira vuto. Tsopano gawo lachiwiri ndikuyamba kuyesa ndi chipangizo chomwe chamalizidwa kale. Mwa izi, cholinga chake ndi kuyesa chipangizocho bwino musanachiyesere mtundu uliwonse. Muyenera kuyang'ana ndikuyang'ana mizere. Mukamayang'ana mizere, pali zinthu zochepa zomwe ziyenera kukumbukiridwa monga kuwona kukhazikika kwa kusonkhana kwa mayeso, kuipitsidwa kwa LFA, komanso ngati pali kuwonongeka kulikonse kwa chipangizocho.

Gawo lotsatira ndikusintha zinthu zomwe zimadziwika kuti zimagwira ntchito posinthanitsa ndi omwe sakugwira ntchito panthawiyo. Pachifukwa ichi, mungafunike kuyang'ana zida zam'mbuyomu ngati vuto likupezeka mu chipangizocho kapena chinthu chilichonse cha LFA. Zomwezo zimachitika ngati mankhwala kapena zinthu zilizonse zomwe zimaganiziridwa kuti ndizomwe zimayambitsa vuto ndiye kuti ziyenera kusinthidwa kuchokera kuzipangizo zam'mbuyomu kuti athane ndi vutoli.

Gawo lotsatira lomwe limabwera munjira iyi ndikufufuza kuthamanga kwa yesero. Kuti mufufuze kuthamanga kwa mayesowo mutha kugwiritsa ntchito kuyesa ndodo. Ndi chithandizo chake, zimakhala zosavuta kuthana ndi mavutowo ngakhale atakhala kuti ndi mankhwala kapena zinthu zachilengedwe. Pogwiritsa ntchito njirayi, kusiyanasiyana kwama pads a conjugate, mapadi oyeserera, nyumba, ndi njira zina zamsonkhano zitha kuthetsedwa. Ndipo mfundo yowonjezerapo ndi kusiyanasiyana kwina kumatha kufufuzidwa nthawi imodzi.

Zinthu zina zomwe zimakhudzidwa mu bukhu lothandizira kuthetsa mavuto la Lateral Flow Assay ndi ogulitsa, opanga ndi QC, ndi ogwiritsa ntchito malonda. Ogulitsa, ali ndi udindo wopanga ubwino ndi kusinthasintha ndikuwonjezera zofunikira zomwe zikugwirizana ndi mayeso a lateral flow. Kuphatikiza apo, njira yopangira ndi kuwongolera khalidwe ziyenera kufufuzidwa chifukwa iyi ndiye sitepe yayikulu komanso yoyambira pakupanga zida zabwino. Maphunziro owonjezereka ayenera kuperekedwa pa njira zokonzedwa bwino komanso khalidwe labwino la malonda. Pomaliza, kwa ogwiritsa ntchito, malangizo ogwiritsira ntchito ayenera kulembedwa momveka bwino komanso momveka bwino kuti amvetsetse kugwiritsa ntchito chipangizocho moyenera.

Mukamagwiritsa ntchito poyeserera kotseguka, pali kuthekera kuti mutha kuwona zotsatira zabodza. Zotsatira zabodza zabodza zimapezeka pomwe wowunika yemwe sanasankhe mu sampuli kapena matrix kulibe koma zotsatira zake zinali zabwino. Kuti tithetse nkhaniyi ndikofunikira kumvetsetsa zomwe zimayambitsa izi. Zifukwazi zitha kukhala zomangika zenizeni, zomangiriza ma heterophilic, kapena kuyambiranso. Pofuna kuthana ndi mavutowa, kukhathamiritsa kwa njira yoyeserera yoyeserera kapena pedi yolumikizira iyenera kuchitidwa. Zomwe tazitchula pamwambazi ndizosiyana kwambiri chifukwa cha kuyandikana, ma antigen, ndi ma antibodies. Komanso kupezeka kwa ma heterophilic antibodies kumatha kubweretsa kulumikizana kwa ma antibayotiki ndi owunika omwe sanayang'anidwe, motero kumadzetsa zotsatira zabodza. Ngati mukuyesa kwanu kotsatira pali ma antibodies a heterophilic mutha kuthana ndi vutoli pogula ma reagents oletsa heterophilic kapena mbewa IgG conjugate itha kugwiritsidwa ntchito kuthana ndi vuto loyeserera lateral.

Vuto lina lomwe lingapezeke pakuyesa kwakanthawi kochepa ndikuchepa kwa mzere woyeserera ndi mzere woyeserera pomwe nyemba kapena masanjidwewo atayikidwa pazitsanzo mu chida cha LFA. Magaziniyi yawonedwa m'milandu ingapo ndipo chifukwa chomwe chimadziwika ndi izi ndizosiyana ndi matrix azachipatala kapena zitsanzo. Pofuna kuthetsa vutoli, kugwiritsa ntchito ma block block mu conjugate diluent kapena conjugation pad kungathandize kuthana ndi vutoli. Osiyanasiyana amatha kukhala mapuloteni, mchere, mamolekyulu, kapena ma metabolites.

Kutsiliza:

Mayeso a Kuyenda kwa Mapazi ndi zida zomwe zimagwiritsidwa ntchito kwambiri padziko lonse lapansi. Komabe, pakhoza kukhala mavuto chifukwa cha zifukwa zosiyanasiyana zomwe munthu angakumane nazo akamagwiritsa ntchito chipangizochi. Buku lothandizira kuthetsa mavuto a kuyesa kuyenda kwa mapazi kumathandiza kuwonetsa zomwe zingachitike pofufuza vutoli ndikulithetsa. Pomaliza pa zonse zomwe zalembedwa pamwambapa, ndikofunikira kunena kuti popanga bwino mayeso oyenda kwa mapazi ndi mizere yoyesera kuyenda kwa mapazi, pali kuphatikiza kwa mavuto achilengedwe, mankhwala, zakuthupi, ndi uinjiniya. Ndikofunikira ndipo tiyenera kuchitapo kanthu kuti tiganizire zinthu zochepa ndi zinthu zina popanga chinthucho kuti tichepetse kusintha kulikonse ngati chipangizocho ndi mizereyo zingapereke zotsatira zolakwika pamapeto pake. Komanso, ngati pali vuto ndi nembanemba ya nitrocellulose ya chipangizo choyesera kuyenda kwa mapazi, vuto likhoza kukhala pakupanga chipangizocho kapena mavuto ena ogwirizana.

Zothandizira

https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf

https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes

https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting