Kutuluka kotsatira kuyesa ndiye mfundo yoyeserera zida zomwe zili ndiukadaulo. Popeza ali ndi zida zamakono, pali mwayi wovuta kapena vuto lomwe lingasokoneze ogwiritsa ntchito kumapeto. Pothetsera vuto, ndikuwunika kwake ndikofunikira kudziwa za kusaka ndondomeko ya Kufufuza Kwotsatira. Lateral Flow Assay itha kukhala ndi zina monga zotsatira zabodza, zotsatira zabodza, zovuta za nembanemba, ndi zina zambiri.
The chitsogozo chazovuta pakuyesa kotsatira zimakhala zofunikira pakakhala chitsimikizo cha kulephera komwe kunanenedwa. Pakulephera komwe akuti, wina ayenera kufotokozera zakulephera pamayeso oyeserera kuti pakhale chidziwitso chokwanira. Kuphatikiza apo, ndikofunikira kudziwa kuchuluka kwakulephera, kuchuluka kwa zomwe zachitika, ndipo chiyerekezo choti kulephera kumeneku kudzachitikanso.
Muupangiri wamavuto, pali masitepe angapo omwe ali ndi njira zina zomwe ziyenera kutsatiridwa ngati nkhaniyo ingachitike ofananira otaya kuyesa mayeso ndi chipangizo. Gawo loyamba ndikufufuza zoyambira za LFA. Pakadali pano, mbiri yakuthupi, ndikusintha kwamayeso a LFA kuyenera kuwunikidwa. Komanso, ngati pali zochitika zosazolowereka pozindikira, zithandizanso kudziwa vuto. Tsopano sitepe yachiwiri ndikuyamba kuyesa ndi chida chatha kale. Mwa ichi, amatanthauza kuyesa chipangizocho bwinobwino musanatenge kuyesedwa kwamtundu uliwonse. Muyenera kuyang'ana ndikuwunika zomwe zidutswazo. Poyang'anitsitsa zolembazo, pali zochepa zofunika kuziganizira monga kuyang'ana kusasunthika kwa mayesowo, kuipitsidwa kwa LFA, komanso ngati pangakhale kuwonongeka kwa chipangizocho.
Gawo lotsatira ndikusintha zinthu zomwe zimadziwika kuti zimagwira ntchito posinthanitsa ndi omwe sakugwira ntchito panthawiyo. Pachifukwa ichi, mungafunike kuyang'ana zida zam'mbuyomu ngati vuto likupezeka mu chipangizocho kapena chinthu chilichonse cha LFA. Zomwezo zimachitika ngati mankhwala kapena zinthu zilizonse zomwe zimaganiziridwa kuti ndizomwe zimayambitsa vuto ndiye kuti ziyenera kusinthidwa kuchokera kuzipangizo zam'mbuyomu kuti athane ndi vutoli.
Gawo lotsatira lomwe limabwera munjira iyi ndikufufuza kuthamanga kwa yesero. Kuti mufufuze kuthamanga kwa mayesowo mutha kugwiritsa ntchito kuyesa ndodo. Ndi chithandizo chake, zimakhala zosavuta kuthana ndi mavutowo ngakhale atakhala kuti ndi mankhwala kapena zinthu zachilengedwe. Pogwiritsa ntchito njirayi, kusiyanasiyana kwama pads a conjugate, mapadi oyeserera, nyumba, ndi njira zina zamsonkhano zitha kuthetsedwa. Ndipo mfundo yowonjezerapo ndi kusiyanasiyana kwina kumatha kufufuzidwa nthawi imodzi.
Zinthu zina zomwe zikukhudzidwa ndi kalozera wamavuto a Kufufuza Kwotsatira ndi ogulitsa, opanga ndi QC, komanso ogwiritsa ntchito kumapeto. Ndi ogulitsa, ali ndi udindo wopanga mtunduwo mosasinthasintha ndikuwonjezera malingaliridwe omwe akukhudzana ndi kuyesa kwazotsatira. Kuphatikiza apo, njira zopangira ndikuwongolera zabwino ziyenera kuwunikidwa popeza ili ndiye gawo lalikulu komanso maziko pakupanga zida zabwino. Maphunziro owonjezera amayenera kuperekedwa pazinthu zoyengedwa komanso mtundu woyenera wa malonda. Pomaliza, kwa ogwiritsa ntchito kumapeto, malangizo ogwiritsira ntchito ayenera kulembedwa momveka bwino komanso momveka bwino kuti amvetsetse kugwiritsa ntchito chipangizocho moyenera.
Mukamagwiritsa ntchito poyeserera kotseguka, pali kuthekera kuti mutha kuwona zotsatira zabodza. Zotsatira zabodza zabodza zimapezeka pomwe wowunika yemwe sanasankhe mu sampuli kapena matrix kulibe koma zotsatira zake zinali zabwino. Kuti tithetse nkhaniyi ndikofunikira kumvetsetsa zomwe zimayambitsa izi. Zifukwazi zitha kukhala zomangika zenizeni, zomangiriza ma heterophilic, kapena kuyambiranso. Pofuna kuthana ndi mavutowa, kukhathamiritsa kwa njira yoyeserera yoyeserera kapena pedi yolumikizira iyenera kuchitidwa. Zomwe tazitchula pamwambazi ndizosiyana kwambiri chifukwa cha kuyandikana, ma antigen, ndi ma antibodies. Komanso kupezeka kwa ma heterophilic antibodies kumatha kubweretsa kulumikizana kwa ma antibayotiki ndi owunika omwe sanayang'anidwe, motero kumadzetsa zotsatira zabodza. Ngati mukuyesa kwanu kotsatira pali ma antibodies a heterophilic mutha kuthana ndi vutoli pogula ma reagents oletsa heterophilic kapena mbewa IgG conjugate itha kugwiritsidwa ntchito kuthana ndi vuto loyeserera lateral.
Vuto lina lomwe lingapezeke pakuyesa kwakanthawi kochepa ndikuchepa kwa mzere woyeserera ndi mzere woyeserera pomwe nyemba kapena masanjidwewo atayikidwa pazitsanzo mu chida cha LFA. Magaziniyi yawonedwa m'milandu ingapo ndipo chifukwa chomwe chimadziwika ndi izi ndizosiyana ndi matrix azachipatala kapena zitsanzo. Pofuna kuthetsa vutoli, kugwiritsa ntchito ma block block mu conjugate diluent kapena conjugation pad kungathandize kuthana ndi vutoli. Osiyanasiyana amatha kukhala mapuloteni, mchere, mamolekyulu, kapena ma metabolites.
Kutsiliza:
Kufufuza Kwotsatira ndizo zida zomwe zimagwiritsidwa ntchito kwambiri komanso padziko lonse lapansi. Komabe, pakhoza kukhala zovuta chifukwa cha zifukwa zosiyanasiyana zomwe munthu angakumane nazo pogwiritsa ntchito chipangizocho. The chitsogozo chazovuta pakuyesa kotsatira Amathandizira kuwonetsa zomwe zingachitike pofufuza vutoli ndikulithetsa. Pomaliza zonse zomwe zalembedwa pamwambapa, ndiyenera kunena kuti pakupanga kuyeserera koyeserera komanso magawo oyeserera ofananira, pali kuphatikiza zinthu zachilengedwe, zamankhwala, zakuthupi, ndi zomangamanga. Ndikulimbikitsidwa ndipo muyenera kuchitapo kanthu kuti muganizire pazinthu zonse zochepa pomwe mukupanga malonda kuti muchepetse mtundu uliwonse wamtunduwu ngati chida ndi zingwe zingabweretse zotsatira zolakwika pamapeto pake. Komanso, ngati pali vuto ndi nembanemba ya nitrocellulose yoyeserera kotsatira kovutikira vutoli lingakhale pakupanga kwa chipangizocho kapena zina pamsonkhano.
Zothandizira
https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf
https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes
https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting
