Otsatira Otsatira Assay ndi chida chomwe chimagwiritsidwa ntchito pamayeso osiyanasiyana pozindikira wowunikira, ngati alipo kapena ayi. Othandizira ofananira Zoyesa zida amagwiritsidwa ntchito padziko lonse lapansi maubwino angapo ndipo umodzi mwamaubwino ake ndikuti atha kugwiritsidwa ntchito poyesa nyumba. Ponena za kuyesa kunyumba, munthu amatha kuzindikira matenda, matenda, komanso kuchita kuyezetsa mimba kunyumba. Kufufuza Kwotsatira Mayesero amagwiritsidwa ntchito kuti azindikire zizindikiro za mkodzo, malovu, magazi, plasma, ndi zitsanzo zina pofuna kufufuza. Ma LFA ochiritsira amakhala ndi zotsatira zochulukirapo kapena zowoneka bwino, zomwe zimafuna kugwiritsa ntchito zida zapadera kuti zizindikire zotsatira za kuchuluka. LFA ndi njira yoyesera yomwe imagwiritsa ntchito pepala kuti izindikire ndikuwerengera ma analytics muzosakaniza zovuta. Chitsanzocho chimayikidwa pa chipangizo choyesera, ndipo zotsatira zake nthawi zambiri zimapezeka mkati mwa mphindi zisanu mpaka makumi atatu.
Zigawo zamagetsi oyenda mozungulira ndi pad pad, conjugate pad, nitrocellulose membrane, ndi adsorbent pad. Chitsanzocho kapena masanjidwewo atayikidwa pachitsanzocho chimayenderera kupita padeti yolumikizira pomwe ma antigen ndi ma antibodies amayamba kumangika ndipo tinthu tating'onoting'ono tomwe timasakanikirana ndi ma nanoparticles omwe ali palagi yolumikizidwa. Kenako, amapita kumtunda. Apa ma nanoparticles amakhala osanthula omangika ndipo pazida za Lateral Flow Assay mizere iwiri ndiyo mzere woyeserera ndi mzere wowongolera. Izi ma nanoparticles ziyamba kudutsa pamzere woyesera kapena mzere wolamulira.
Mzere woyeserera uli ndi ma nanoparticles osasunthika omwe amatha kuphatikiza ndi tinthu tating'onoting'ono tomwe timasanjidwa posonyeza kukhalapo kwa wowunikira. Komabe, kuchokera pamzere woyesera, madzimadzi amapita kumzere wowongolera. Mzere wolamulira umakhala ndi maubwenzi oyandikana omwe amagwira ntchito kuti amange tinthu tomwe timagwirizana ndi wowunikira kapena wopanda wowunikira kuti awonetse kuti chida choyesa cha lateral chikuyenda bwino komanso moyenera. Tsopano, madzi atadutsa kuchokera mizere yonse mwachitsanzo mzere woyeserera ndi mzere wolamulira pamakhala cholembera cha adsorbent. Adsorbent pad imagwira ntchito yotsatsa zakumwa zonse zowonjezerazo. Popeza pamafunika masanjidwe ochepa ndi owongoleredwa, chifukwa chake, amaonetsetsa kuti madzi akuyenda omwe amafunikira kokha.
Pochita mayeso oyembekezera pa zipangizo lateral otaya awa ndi mitundu iwiri mwachitsanzo mtundu wopikisana ndi mtundu wa sangweji. Kugwiritsa ntchito mtundu wopikisana ndiko kuzindikira ma analytiki ngati ma antibodies kapena analyte sakukwanira kulumikizana kangapo. Pamipikisano, pali ma molekyulu a analyte pamzere woyeserera komanso zovuta zowonera mapuloteni omwe ali ndi phukusi la conjugate lomwe limafotokozera za kupezeka kapena kusowa kwa womasulira. Mumtundu wamtunduwu, ngati wowunikira sangapezeke, ma conjugates adzafika pamzere woyeserera. Kubwera ku sangweji yamayeso oyeserera ofananira ndi zida zoyembekezera, mtunduwu umagwiritsidwa ntchito pozindikira ma analytiki omwe akufuna, ngati alipo kapena ayi, Ma analytics akuluakuluwa ayenera kukhala ndi ma epitopes. Komanso ma antibodies omwe ali m'malo awiri oyimba amtunduwu amamanga m'njira yomwe katswiri wina amamangirira ndi tinthu tomwe taphatikizana ndikuwunika kwinanso ndi mayeso ena omanga omwe adzagwiritsidwe ntchito poyesa mzere wa Assay. Komabe, ngati wofufuzayo atapezeka adzalumikizana ndi anti-nanoparticle conjugate ndikupereka zotsatira zomwe akufuna. Komanso, mfundo yofunika ndiyakuti mumapangidwe a sangweji wa chida chotsatira cha Latera Flow pakuyesa mimba, mzere wachikuda ukuwoneka kuti zikutanthauza kuti zotsatirazo ndi zabwino, Komabe, pamipikisano padzakhala kupezeka / kusowa kwa mizere yachikuda pa chipangizocho chomwe chimatanthauza kuti zotsatira zake ndi zabwino. muchida chofufuzira cha lateral chimagwira ntchito motere poyezetsa pakati.
Pakuwunika kwa kuyesa kwa lateral flow assay test, ndikofunikira kudziwa kuti mayeserowa atha kukhala pazizindikiro zomwe amatha kuwerengera ndi maso ndipo zimamveka mosavuta. Kuti muwonetsetse kukhudzika kwa chida choyesa chotsatira ndikofunikira kukhala ndi chizindikiro champhamvu kwambiri chomwe chingatheke pamlingowo. Ngati pali tinthu tating'onoting'ono tomwe kumangirako kungakhale kolimba koma ngati kungakhale kokulirapo padzakhala choletsa poyeserera. Zotsatira zakapangidwe kazoyeserera kotsogola zitha kukhala munjira zotsatirazi, mwachitsanzo, zoyenerera, zoyeserera, komanso zochulukirapo. Komabe, mayeso amimba pamagwiritsidwe oyeserera ofananira nawo, adzawonetsa zotsatira zabwino mwachitsanzo Inde kapena Ayi Kuyesa Kwakutsogolo Kwa Mimba, kusanthula ndi zotsatira zake kutengera HCG kusanthula. HCG imayimira Human chorionic gonadotropin yomwe imapangidwa ndi maselo a trophoblast. Matrix ikaikidwa pachitsanzo cha chipangizocho, Human chorionic gonadotropin imamangidwa ndi ma nanoparticles agolide omwe amaphatikizidwa ndi anti antibody ndi conjugated antibody. Chifukwa awa ndi kuphatikiza kwa hCG mumkodzo wanu, kuchuluka kwake kumasiyana kutengera mukamayesa mayeso. Malire oyesa mayeso nthawi zambiri amakhala pakati pa 20 ndi 100 mIU / mL. Kulankhula za kuchuluka kwazidziwitso, kulimbikira kwa mzere woyeserera kumatsimikiziridwa ndi muyeso womwe umatchedwa calibration standard ndipo mu ichi, ma analytic amasinthidwa kukhala mtengo woyikira. Kuti mudziwe zotsatira zolondola, tikulimbikitsidwa kuti mugwiritse ntchito zowerengera kuti muwerenge zotsatira pazotsatira za Flow Assay.
Kutsiliza
Othandizira ofananira Zoyesa zida ndi okwera mtengo komanso osavuta kugwiritsa ntchito zida zomwe zikugwiritsidwa ntchito ndi mayiko otukuka komanso akutukuka. Kuphatikiza apo, zida zoyendera pambuyo pake ndi imodzi mwanjira zodalirika zoyeserera za mayesero apakati chifukwa amapereka zotsatira zowona ndipo pafupifupi aliyense amadziwa momwe angawagwiritsire ntchito mosavuta. Chida cha Lateral Flow Assay ndipamwamba kwambiri pakuyesa mtundu uliwonse kunyumba.
Zothandizira
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4
