Kuyesa Kwakuyenda Pakati Pathupi Momwe imagwirira ntchito?
Kuyesa kwa Lateral Flow ndi chipangizo chomwe chimagwiritsidwa ntchito poyesa mitundu yosiyanasiyana pozindikira analyte yofunikira, ngati ilipo kapena ayi mu chitsanzo. Zipangizo zoyesera za Lateral Flow zimagwiritsidwa ntchito padziko lonse lapansi pazabwino zingapo ndipo chimodzi mwazabwino zake ndichakuti zitha kugwiritsidwa ntchito poyesa kunyumba. Ponena za kuyesa kunyumba, munthu amatha kuzindikira matenda, matenda, komanso kuchita mayeso a mimba kunyumba . Mayeso a Lateral Flow Assay amagwiritsidwa ntchito kuzindikira zizindikiro mu mkodzo, malovu, magazi, plasma, ndi zitsanzo zina kuti azindikire matenda. Ma LFA achizolowezi amapereka zotsatira za semiquantitative kapena qualitative, zomwe zimafuna kugwiritsa ntchito zida zapadera kuti azindikire zotsatira za kuchuluka. LFA ndi njira yoyesera yomwe imagwiritsa ntchito pepala kuti izindikire ndikuwerengera ma analytes m'zosakaniza zovuta. Chitsanzocho chimayikidwa pa chipangizo choyesera, ndipo zotsatira zake nthawi zambiri zimapezeka mkati mwa mphindi zisanu mpaka makumi atatu.
Zigawo zamagetsi oyenda mozungulira ndi pad pad, conjugate pad, nitrocellulose membrane, ndi adsorbent pad. Chitsanzocho kapena masanjidwewo atayikidwa pachitsanzocho chimayenderera kupita padeti yolumikizira pomwe ma antigen ndi ma antibodies amayamba kumangika ndipo tinthu tating'onoting'ono tomwe timasakanikirana ndi ma nanoparticles omwe ali palagi yolumikizidwa. Kenako, amapita kumtunda. Apa ma nanoparticles amakhala osanthula omangika ndipo pazida za Lateral Flow Assay mizere iwiri ndiyo mzere woyeserera ndi mzere wowongolera. Izi ma nanoparticles ziyamba kudutsa pamzere woyesera kapena mzere wolamulira.
Mzere woyeserera uli ndi ma nanoparticles osasunthika omwe amatha kuphatikiza ndi tinthu tating'onoting'ono tomwe timasanjidwa posonyeza kukhalapo kwa wowunikira. Komabe, kuchokera pamzere woyesera, madzimadzi amapita kumzere wowongolera. Mzere wolamulira umakhala ndi maubwenzi oyandikana omwe amagwira ntchito kuti amange tinthu tomwe timagwirizana ndi wowunikira kapena wopanda wowunikira kuti awonetse kuti chida choyesa cha lateral chikuyenda bwino komanso moyenera. Tsopano, madzi atadutsa kuchokera mizere yonse mwachitsanzo mzere woyeserera ndi mzere wolamulira pamakhala cholembera cha adsorbent. Adsorbent pad imagwira ntchito yotsatsa zakumwa zonse zowonjezerazo. Popeza pamafunika masanjidwe ochepa ndi owongoleredwa, chifukwa chake, amaonetsetsa kuti madzi akuyenda omwe amafunikira kokha.
Pochita mayeso a mimba pa zipangizo zoyendera mbali zomwe zili ndi mawonekedwe awiri monga mawonekedwe opikisana ndi mawonekedwe a sandwich. Kugwiritsa ntchito mawonekedwe opikisana ndi njira yopezera ma analyte ngati ma antibodies kapena analyte sakwanira kuti ma antibodies kapena analyte agwirizane ndi ma multiple binding. Mu mtundu wopikisana, pali ma analyte molecules mu mzere woyesera ndi protein-analyte complex yokhala ndi conjugate pad yomwe imasonyeza kupezeka kapena kusakhalapo kwa analyte. Mu mtundu uwu wa mawonekedwe, ngati analyte yolunjika palibe, ma conjugates amamangirira ku mzere woyesera. Ponena za mawonekedwe a sandwich a lateral flow assay devices, mawonekedwe awa amagwiritsidwa ntchito pozindikira ma target analytes, ngati alipo kapena ayi. Kuphatikiza apo, ma analytes akuluakulu awa ayenera kukhala ndi ma epitopes. Komanso ma antibodies m'malo awiri oyesera amtunduwu amamangirira mwanjira yomwe analyte imodzi imalumikizana ndi tinthu tomwe talumikizidwa ndi analyte ina ndi mayeso ena omangirira omwe adzagwiritsidwa ntchito pa mzere woyesera wa lateral flow Assay. Komabe, ngati katswiri wofufuza matenda apezeka, amalumikizana ndi antibody-nanoparticle conjugate ndipo amapereka zotsatira zomwe mukufuna. Komanso, mfundo yofunika ndi yakuti mu mtundu wa sandwich wa chipangizo cha latera Flow essay cha mayeso a mimba, mzere wamitundu umawonekera zomwe zikutanthauza kuti zotsatira zake ndi zabwino. Komabe, mu mtundu wopikisana padzakhala kusowa/kusowa kwa mizere yamitundu pa chipangizocho zomwe zikutanthauza kuti zotsatira zake ndi zabwino. mu chipangizo choyesera matenda a lateral flow chimagwira ntchito motere pochita mayeso a mimba pa icho.
Pakuwunika mayeso a lateral flow assay, ndikofunikira kudziwa kuti mayesowo amakhala m'zizindikiro zomwe zingawerengedwe ndi maso ndipo zimamveka mosavuta. Kuti muwonetsetse kuti chipangizo choyesera lateral flow assay chili ndi mphamvu, ndikofunikira kukhala ndi chizindikiro champhamvu kwambiri chomwe chingatheke. Ngati pali tinthu tating'onoting'ono tomwe timalumikizana timakhala olimba koma ngati titakhala tambiri kwambiri padzakhala choletsa kuyenda kwa chitsanzo. Zotsatira za chipangizo choyesera lateral flow zidzakhala m'njira zotsatirazi monga qualitative, semi-qualitative, ndi quantitative. Komabe, mayeso a mimba pa chipangizo choyesera lateral flow assay, adzawonetsa zotsatira za qualitative mwachitsanzo Inde kapena Ayi. Mu Lateral Flow Pregnancy Test , kusanthula ndi zotsatira zake zimachokera ku HCG analysis. HCG imayimira Human chorionic gonadotropin yomwe imapangidwa ndi maselo a trophoblast. Pamene matrix iikidwa pa sample pad ya chipangizocho, Human chorionic gonadotropin imalumikizidwa ku nanoparticles zagolide zomwe zimaphatikizidwa ndi test antibody ndi conjugated antibody. Popeza uku ndi kusonkhana kwa hCG mu mkodzo wanu, kuchuluka kwake kumasiyana malinga ndi nthawi yomwe mwatenga mayesowo. Malire ozindikira mayeso nthawi zambiri amakhala pakati pa 20 ndi 100 mIU/mL. Ponena za kuchuluka kwa matenda, kuchuluka kwa mzere woyesera kumatsimikiziridwa ndi muyezo wokhazikitsidwa womwe umatchedwa muyezo wowerengera ndipo mu izi, ma analyte amasinthidwa kukhala kuchuluka kwa kuchuluka. Kuti mudziwe zotsatira zolondola, tikukulimbikitsani kugwiritsa ntchito chowerengera cha strip kuti muwerenge zotsatira pa chipangizo cha Lateral Flow Assay.
Kutsiliza
Zipangizo zoyesera za Lateral Flow Assay ndi zotsika mtengo komanso zosavuta kugwiritsa ntchito zomwe zikugwiritsidwa ntchito ndi mayiko otukuka ndi osauka. Kuphatikiza apo, zipangizo zoyesera za lateral flow ndi imodzi mwa njira zodalirika kwambiri zoyesera mimba chifukwa zimapereka zotsatira zenizeni ndipo pafupifupi aliyense amadziwa momwe angagwiritsire ntchito nthawi iliyonse yomwe angafune. Zipangizo zoyesera za Lateral Flow Assay ndi zapamwamba kwambiri pochita mayeso aliwonse kunyumba.
Zothandizira
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4