Otsatira Otsatira Assay, chida kapena zoyeserera zoyesa kudziwa kupezeka kapena kupezeka kwa wowunika muzoyeserazo, ali ndi makina omwe amatsatiridwa pakuyesedwa konse. Makinawa amatsimikizira kuyeserera kwa mayeso, kutsatira njira, ndikupeza zotsatira za mayeso. Kuphatikiza apo, ziyenera kudziwika kuti ngakhale ukadaulo wakhala paliponse pachipatala ndipo Kufufuza Kwotsatira sali olekanitsidwa ndi izo. Chifukwa chake, ukadaulowu waphatikizidwa mu Kufufuza Kwotsatira koma momwe amagwirira ntchito kapena makina awo amakhalabe ofanana. Ndipo ichi ndi chimodzi mwazifukwa zomwe ndicho chida choyesera kwambiri kumayiko otukuka komanso akutukuka.
Kuti timvetse momwe makina a Kufufuza Kwotsatira, nayi mwachidule mwachidule momwe imagwirira ntchito. Choyambirira, pali padi yoyeserera yomwe tsengalo limayikidwa, ndipo limapitanso padongosolo la conjugate. Pazipangizo zogwiritsira ntchito conjugate, zochitika zomangika zimachitika chifukwa chakupezeka kwa tinthu tomwe tikhoza kukhala golide kapena latex. Chotsatira chomwe chimachitika ndikuti woyeserera ndi wothandizira amamangirira ndikusunthira ku nembanemba. Kakhungu kamene kamathandizira pakupanga zotsatira. Mzere woyeserera umayima kapena ukupitilira kuyenda mpaka pad adsorbent. Kuti mudziwe ngati Lateral Flow Assay idagwira othandizira omwe amathandizira amamangirira pamzere wolamulira ndikupanga zotsatira. Makina atsatanetsatane a Lateral Flow Assay afotokozedwa motsatira.
Zitsanzo Pad ndi kuyamba kwa mayeso ofananira otaya mayeso. Kapangidwe kazomwe amagwiritsa ntchito pad ndi fiber fiber, mauna oluka, kapena mapadi. CHIKWANGWANI chamagalasi, mauna oluka, ndi mapadi ali ndi zinthu zosiyanasiyana kuchokera kwa wina ndi mnzake. Komanso, kugwira ntchito ndi mtengo wawo kumadaliranso mtundu. Kwenikweni, pulogalamu yoyesererayo imayambitsa kuyesa kwa Lateral Flow Assay ndipo ntchito yayikulu ndikupeza kuchuluka kwa chitsanzocho kapena chitsanzocho ndikugawa. Ndikofunikira kuti phukusi lachitsanzo lilole kuti nyembazo zisasunthike kuti zithe kupatukana ndi nyerereyo, kusintha kwa pH, ndikumanga, wogawana. Kuti mayeso ayambidwe, chitsanzocho chiyenera kuyikidwa papepala loyeserera.
Pedi ya conjugate ndiye gawo lachiwiri la makina a Lateral Flow Assay. Conjugate Release Pad imakhala ndi magalasi osalukidwa osalala ndipo pamakhala wothandizira yemwe wayanika kale. Kulumikiza ndi pulogalamu yoyeserera, pomwe nyerereyo ikafika mpaka kukhathamira ndikutsikira padothi lotulutsira conjugate ndipo apa wothandizirayo atulutsidwa kuti awunikire chitsanzocho. Ntchito yayikulu ya phukusi la conjugate ndikuwonetsetsa kuti chitsanzocho ndi wothandizirayo asunthidwa ku nembanemba ndikusunganso conjugate. Pazipangizo za conjugate, payenera kukhala zomangika zosatsimikizika kuti zitsimikizire kuti reagent siyokhazikika pachipindacho.
Nthawi zonse pamakhala kupezeka kwa reagent mu ofananira otaya kuyesa mayeso. Reagent yodziwika ili ndi ma antibodies omwe amaphatikizidwanso ndi fulorosenti kapena tinthu tating'onoting'ono. Kuchita bwino kwa mayesero oyenda mozungulira kumadalira mtundu wa ma antibodies ndi reagent reagent.
Kusankhidwa kwa antibody woyeserera poyeserera poyeserera kumawerengedwa kuti ndikofunikira ndipo ndi zinthu zochepa zomwe ziyenera kuganiziridwa posankha antibody woyeserera pambuyo pake. Choyambirira ndikuti mupezere chitetezo cha mthupi. Kachiwiri, kutulutsa kwa antigen kuyenera kufufuzidwa ndikutsimikizika. Komanso, kusankha pakati pa monoclonals kapena polyclonals posankha ma antibodies. Ma monoclonals amalimbikitsidwa kuti pakhoza kukhala kupanga ma antibodies ambiri ndipo potero amathandizira kuzindikira epitope imodzi yama antigen pomwe ma Polycilonals amatha kukhala osinthika ndipo kupezeka kwawo sikutsimikiziridwa mwina.
Kakhungu Kakhungu kapena Nitcocellulose Membrane ndichimodzi mwazinthu zofunikira kwambiri pakuwunika kwa Lateral Flow Assay komanso kumvetsetsa kwake. Mzere woyeserera ndi mzere wowongolera umakokedwa pamwamba pa nembanemba kuti ikhale yolumikizana bwino kuti ipeze ma antibodies. Kutsatsa kwapadera pamizere yoyesa ndi mizere yolamulira kumatha kukhudza chidwi cha zotsatira zomwe zingabweretse zotsatira zabodza.
Pad adsorbent Pad kapena Wicking pad ili kumapeto kwa mzere kapena chida chotsatira chotsatira. Cholinga cha phukusi lokulira chikhoza kukhala ndi fyuluta ya cellulose yomwe helios pokhathamiritsa kuchuluka kwa chitsanzocho pamayeso oyeserera. Ntchito yayikulu ya adsorbent pad ndikuwongolera mayendedwe amadzimadzi pamtundu wa nitrocellulose. Osati zokhazo, koma imayimitsanso kubwerera m'mbuyo kwa nyembazo motero kukhalabe ndi chitsanzocho kapena chitsanzocho.
Ngakhale zochita mwatsatanetsatane kapena makinawo zafotokozedwa mwatsatanetsatane, njira zomwe zingachitike poyeserera kotsogola ndizofunikira kwa izo. Pali njira ziwiri zomwe mungasankhe; imodzi ikukonzekera batch ndipo inayo ili pamzere wapa-line / reel-to-reel. Kukonza magulu ndiwothandiza kwambiri ndipo kumafunikira ogwira ntchito omwe angapangitse kuti zisinthe pakhale zotsatira, ndikupanga zotsatira zosadalirika. Pomwe kusonkhana kwa reel-to-reel ndimakina osinthidwa osankhidwa ndi odalirika kwambiri pakukula kwakukulu kapena kwakukulu. Pakati pa mitundu iwiri yonse yazosankha, mawonekedwe a kutentha ndiofunika kwambiri ndipo amayenera kuwonedwa ngati akupereka zowona komanso zofunika.
The Njira Yotsatira Yoyeserera Ili ndi magawo ochepa omwe amaphatikizira mapadi, mamina, ndi tinthu tina tomwe timathandiza kuzindikira mtunduwo ndikupanga zotsatira. Ngakhale pali zida zosiyanasiyana za Lateral Flow Assay ndipo zina mwazo zimapangidwa mwaluso pomwe zina sizili choncho koma makinawo amakhalabe ofanana. Zinthu zonse zomwe zimayendetsa Lateral Flow Assay ziyenera kuonedwa kuti ndizofunikira ndipo siziyenera kunyalanyazidwa.
Zothandizira
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test
