Isiphelo soVavanyo lweSisombululo seengxaki

Uvavanyo lokuhamba kwecala luphambili lwezixhobo zokuvavanya ukhathalelo ezixhotyiswe ngobuchwepheshe. Njengoko zixhotyiswe ngobuchwepheshe, kukho ithuba lokuba kubekho nayiphi na ingxaki okanye impazamo enokubaphazamisa ngakumbi abasebenzisi bayo bokugqibela. Ukuze kulungiswe ingxaki, kunye nohlalutyo lwayo kubalulekile ukwazi ngenkqubo yokusombulula ingxaki yeLateral Flow Assay . Uvavanyo lokuhamba kwecala lunokuba neengxaki ezithile ezifana neziphumo ezingalunganga, iziphumo ezingalunganga, iingxaki ze-membrane, kunye nezinye ezininzi.

Isikhokelo sokusombulula iingxaki zovavanyo lokuhamba kwecala siba yimfuneko xa kukho ubungqina bokusilela okuxeliweyo. Kwimpazamo exeliweyo, umntu kufuneka achaze ukungaphumeleli kuvavanyo lokuhamba kwecala ukuze kubekho ulwazi olwaneleyo. Ngaphezu koko, kubalulekile ukwazi ukuba kwenzeka kangaphi ukusilela, inani lezihlandlo ezenzekileyo, kunye nokuba loluphi umlinganiselo wokungaphumeleli okunokwenzeka kwakhona.

Kwisikhokelo sokusombulula iingxaki, kukho amanyathelo amaninzi anamanyathelo angaphezulu ekufuneka alandelwe xa kuvela ingxaki kuvavanyo lwe -lateral flow assay kunye nesixhobo. Inyathelo lokuqala kukuphanda iziseko ze-LFA. Kweli nyathelo, imbali yezinto ezibonakalayo, kunye nokuguquguquka kwenkqubo yovavanyo lwe-LFA kufuneka kujongwe. Kwakhona, ukuba kukho ukubonwa okungaqhelekanga okuqatshelweyo ngelixa kusenziwa uvavanyo, kuya kuba luncedo ekuchongeni ingxaki. Ngoku inyathelo lesibini kukuqala uvavanyo ngesixhobo esele sigqityiwe. Oku, kwenzelwe ukuvavanya isixhobo kakuhle ngaphambi kokuba usithathele naluphi na uhlobo lovavanyo. Kufuneka ujonge kwaye uhlole imicu. Ngelixa uhlola imicu, kukho izinto ezimbalwa ekufuneka zikhunjulwe ezifana nokujonga ukuhambelana kokuhlanganiswa kwemicu, ungcoliseko kwi-LFA, kunye nokuba kukho nawuphi na umonakalo emzimbeni kwisixhobo.

Isinyathelo esilandelayo sithatha indawo yezinto eziyaziwayo ngokusebenza ngokutshintshiselana nalabo abangasebenzi ngelo xesha. Ukulungiselela le njongo, unokufuna ukukhangela iiseti zangaphambili zezixhobo ukuba ingxaki efumanekayo ikwisixhobo okanye nayiphi na into ethile yesixhobo seLFA. Kukwanjalo nakwimeko yayo nayiphi na imichiza okanye izinto zebhayoloji ekucingelwa ukuba zingunobangela wengxaki emva koko kufuneka zibekwe endaweni yebhetshi yangaphambili yezixhobo ukoyisa le ngxaki.

Inyathelo elilandelayo eliza kule nkqubo kukuphanda isantya se-assay. Ukuphanda ngesantya se-assay ungasebenzisa i-ass-stick stick. Ngoncedo lwayo, kuya kuba lula ukusombulula imiba nokuba zizinto zamachiza okanye izinto zebhayoloji. Ngokusebenzisa le ndlela, umahluko kwiipads zokudibanisa, iisampulu zepads, izindlu, kunye neenkqubo ezinxulumene nendibano zinokupheliswa. Kwaye inqaku lokudibanisa zezinye izinto ezinokukhangelwa ngaxeshanye.

Ezinye izinto ezibandakanyekayo kwisikhokelo sokusombulula iingxaki zeLateral Flow Assay ngabathengisi, abavelisi kunye ne-QC, kunye nabasebenzisi bokugqibela bemveliso. Ngabathengisi, banoxanduva lokuvelisa umgangatho ngokuhambelana kunye nokongeza iinkcukacha ezifanelekileyo kuvavanyo lokuhamba kwecala. Ngaphezu koko, inkqubo yokuvelisa kunye nolawulo lomgangatho kufuneka ihlolwe njengoko eli linyathelo eliphambili nelisisiseko lokuvelisa izixhobo ezisemgangathweni. Uqeqesho oluphuculweyo kufuneka lunikwe kwiinkqubo ezicociweyo kunye nomgangatho ocociweyo wemveliso. Okokugqibela, kubasebenzisi bokugqibela, imiyalelo yokusebenza kufuneka ibhalwe ngokucacileyo nangokucacileyo ukuze baqonde ukusebenzisa isixhobo ngendlela enoxanduva.

Ngelixa usebenzisa i-assortal ye-lateral flow, kunokwenzeka ukuba ubone iziphumo ezinobuxoki. Iziphumo eziqinisekileyo zobuxoki zenzeka xa umhlalutyi onqwenelekayo kwisampulu okanye kwimatrix engekho kodwa iziphumo zaphuma zilungile. Ukuyicombulula le ngxaki kubalulekile ukuba uqonde izizathu ezahlukeneyo ezingasemva kwayo. Izizathu zinokubopha okungabalulekanga, ukubopha i-heterophilic, okanye ukuphinda-phinde wenze umsebenzi kwakhona. Ukoyisa le micimbi, ukwenziwa kwe-assay yokuhamba ngokulandelelana okanye iphedi yokudibanisa kufuneka yenziwe. Oonobangela abakhankanywe apha ngasentla bahluke ngokupheleleyo omnye komnye ngokubhekisele kubumbano, iantigen, kunye neentsholongwane. Kwakhona, ubukho be-heterophilic antibodies bunokukhokelela ekubophweni kwee-antibodies kunye nohlalutyo olungakhange lujolise, kungoko kukhokelela kwiziphumo ezingezizo. Ukuba kulungelelwaniso lwakho lokuhamba ngokulandelelana kukho ii-antibacteria ze-heterophilic onokuthi uwusombulule lo mbandela ngokuthenga i-heterophilic blocking reagents okanye i-mouse ye-IgG conjugate ingasetyenziselwa ukusombulula lo mbandela we-assal flow assay.

Enye ingxaki enokufumaneka kwi-lateral flow assay kukuncipha kolawulo kunye nomgca wovavanyo xa isampulu okanye imatriki ifakwa kwiphedi yesampulu kwisixhobo se-LFA. Lo mbandela uye waqwalaselwa kumatyala aliqela kwaye isizathu esichongiweyo zezi zizinto ezahlukeneyo kwimatriki yeklinikhi okanye kwisampulu. Ukusombulula le ngxaki, ukusetyenziswa kweearhente zokuthintela kwi-conjugate diluent okanye kwi-conjugation pad kunokunceda ukoyisa le ngxaki. Iiarhente ezahlukeneyo zokuthintela kunokuba ziiproteni, ityuwa, iimolekyuli, okanye imetabolites.

Isiphelo:

IiLateral Flow Assays zezona zixhobo zisetyenziswa kakhulu kwihlabathi liphela. Nangona kunjalo, kunokubakho iingxaki ngenxa yezizathu ezahlukeneyo umntu anokujongana nazo ngelixa esebenzisa isixhobo. Isikhokelo sokusombulula iingxaki zovavanyo lokuhamba kwecala sinceda ukubonisa oko kunokwenziwa ukuhlalutya ingxaki nokuyisombulula ngakumbi. Ukuqukumbela kuzo zonke izinto ezibhalwe apha ngasentla, kufuneka kuthiwe ukuze kwenziwe ngempumelelo uvavanyo lokuhamba kwecala kunye nemicu yovavanyo lokuhamba kwecala, kukho indibaniselwano yeengxaki zebhayoloji, zekhemikhali, zefiziksi, kunye nezobunjineli. Kuyacetyiswa kwaye kufuneka kuthathwe inyathelo lokuqwalasela zonke izinto ezincinci kunye nemiba ngelixa kusenziwa imveliso ukunciphisa naluphi na uhlobo lotshintsho ukuba isixhobo kunye nemicu zinokuvelisa iziphumo ezingalunganga ekugqibeleni. Kwakhona, ukuba kukho ingxaki nge-nitrocellulose membrane yesixhobo sovavanyo lokuhamba kwecala, ingxaki inokuba kuyilo lwesixhobo okanye eminye imiba yokuhlanganisa.

Ucaphulo

https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf

https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes

https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting