Isiphelo soVavanyo lweSisombululo seengxaki

Ukuhamba kwexesha iimvavanyo ziindawo zovavanyo lokhathalelo ezixhotyiswe ngezobuchwephesha. Njengoko bexhotyisiwe ngezobuchwephesha, kukho ithuba layo nayiphi na ingxaki okanye isiphoso esinokubangela ukubadida abasebenzisi bayo. Isisombululo sengxaki, kunye nohlalutyo lwayo kubalulekile ukwazi malunga Inkqubo yokusombulula ingxaki Ukuvavanywa kokuhamba kamva. Ukuvavanywa kokuhamba ngokuhamba kwexesha kunokuba nemicimbi efana neziphumo eziqinisekileyo zobuxoki, iziphumo ezingezizo zobuxoki, imiba yeembumba kunye nezinye ezininzi.

The Isikhokelo seengxaki kwi-assay flow lateral iba yimfuneko xa kukho ubungqina bokusilela okuxeliweyo. Ukusilela okuxeliweyo, umntu makachaze ukusilela kuvavanyo lokuvavanywa kokuhamba ngokulandelelana ukuze kubekho ulwazi olwaneleyo. Ngaphaya koko, kubalulekile ukwazi amaxesha okusilela, inani lamaxesha okwenzekileyo, kwaye nguwuphi umlinganiso wokungaphumeleli oza kwenzeka kwakhona.  

Kwisikhokelo sokujongana neengxaki, kukho amanyathelo aliqela anamanyathelo amanye ekufuneka elandelwe kwimeko apho kuvela umba Uvavanyo lokuvavanywa kokuhamba kamva kunye nesixhobo. Inyathelo lokuqala kukuphanda iziseko ze-LFA. Kweli nyathelo, kufuneka kutshekishwe imbali yezinto, kunye nenkqubo eyahlukileyo yovavanyo lwe-LFA. Kwakhona, ukuba kukho ukuqaphela okungaqhelekanga kuqatshelwe ngelixa kusenziwa uvavanyo, ziya kuba luncedo nasekuchongeni ingxaki. Ngoku inyathelo lesibini kukuqala kovavanyo ngesixhobo esele sigqityiwe. Ngale nto, yenzelwe ukuvavanya isixhobo ngokufanelekileyo ngaphambi kokuthatha naluphi na uhlobo lovavanyo. Kuya kufuneka ujonge kwaye uhlole imitya. Ngelixa ujonga imitya, zimbalwa izinto eziqwalaselweyo ekufuneka zigcinwe engqondweni njengokujonga ukungqinelana kwendibano yeemvavanyo, ungcoliseko kwi-LFA, kwaye ukuba kukho nawuphi na umonakalo emzimbeni kwisixhobo.

Isinyathelo esilandelayo sithatha indawo yezinto eziyaziwayo ngokusebenza ngokutshintshiselana nalabo abangasebenzi ngelo xesha. Ukulungiselela le njongo, unokufuna ukukhangela iiseti zangaphambili zezixhobo ukuba ingxaki efumanekayo ikwisixhobo okanye nayiphi na into ethile yesixhobo seLFA. Kukwanjalo nakwimeko yayo nayiphi na imichiza okanye izinto zebhayoloji ekucingelwa ukuba zingunobangela wengxaki emva koko kufuneka zibekwe endaweni yebhetshi yangaphambili yezixhobo ukoyisa le ngxaki.

Inyathelo elilandelayo eliza kule nkqubo kukuphanda isantya se-assay. Ukuphanda ngesantya se-assay ungasebenzisa i-ass-stick stick. Ngoncedo lwayo, kuya kuba lula ukusombulula imiba nokuba zizinto zamachiza okanye izinto zebhayoloji. Ngokusebenzisa le ndlela, umahluko kwiipads zokudibanisa, iisampulu zepads, izindlu, kunye neenkqubo ezinxulumene nendibano zinokupheliswa. Kwaye inqaku lokudibanisa zezinye izinto ezinokukhangelwa ngaxeshanye.

Eminye imiba ebandakanyekayo kwisikhokelo sokujongana neengxaki ze Ukuvavanywa kokuhamba kamva ngabathengisi, abemveliso kunye neQC, kunye nabasebenzisi bokugqibela bemveliso. Ngabathengisi, banoxanduva lokuvelisa umgangatho ngokungaguquguquki kunye nokongeza iinkcukacha ezichaphazelekayo kulingo lokuhamba ngokulandelelana. Ngapha koko, inkqubo yokuvelisa kunye nolawulo lomgangatho kufuneka ihlolwe kuba eli lelona nyathelo liphambili nelisisiseko kwimveliso yezixhobo ezisemgangathweni. Uqeqesho oluphuculweyo kufuneka lunikezwe kwiinkqubo ezicokisekileyo kunye nomgangatho ococekileyo wemveliso. Okokugqibela, kubasebenzisi bokugqibela, imiyalelo yokusebenza kufuneka ibhalwe ngokucacileyo nangokucacileyo ukuze baqonde ukusebenzisa isixhobo ngendlela efanelekileyo.

Ngelixa usebenzisa i-assortal ye-lateral flow, kunokwenzeka ukuba ubone iziphumo ezinobuxoki. Iziphumo eziqinisekileyo zobuxoki zenzeka xa umhlalutyi onqwenelekayo kwisampulu okanye kwimatrix engekho kodwa iziphumo zaphuma zilungile. Ukuyicombulula le ngxaki kubalulekile ukuba uqonde izizathu ezahlukeneyo ezingasemva kwayo. Izizathu zinokubopha okungabalulekanga, ukubopha i-heterophilic, okanye ukuphinda-phinde wenze umsebenzi kwakhona. Ukoyisa le micimbi, ukwenziwa kwe-assay yokuhamba ngokulandelelana okanye iphedi yokudibanisa kufuneka yenziwe. Oonobangela abakhankanywe apha ngasentla bahluke ngokupheleleyo omnye komnye ngokubhekisele kubumbano, iantigen, kunye neentsholongwane. Kwakhona, ubukho be-heterophilic antibodies bunokukhokelela ekubophweni kwee-antibodies kunye nohlalutyo olungakhange lujolise, kungoko kukhokelela kwiziphumo ezingezizo. Ukuba kulungelelwaniso lwakho lokuhamba ngokulandelelana kukho ii-antibacteria ze-heterophilic onokuthi uwusombulule lo mbandela ngokuthenga i-heterophilic blocking reagents okanye i-mouse ye-IgG conjugate ingasetyenziselwa ukusombulula lo mbandela we-assal flow assay.

Enye ingxaki enokufumaneka kwi-lateral flow assay kukuncipha kolawulo kunye nomgca wovavanyo xa isampulu okanye imatriki ifakwa kwiphedi yesampulu kwisixhobo se-LFA. Lo mbandela uye waqwalaselwa kumatyala aliqela kwaye isizathu esichongiweyo zezi zizinto ezahlukeneyo kwimatriki yeklinikhi okanye kwisampulu. Ukusombulula le ngxaki, ukusetyenziswa kweearhente zokuthintela kwi-conjugate diluent okanye kwi-conjugation pad kunokunceda ukoyisa le ngxaki. Iiarhente ezahlukeneyo zokuthintela kunokuba ziiproteni, ityuwa, iimolekyuli, okanye imetabolites.

Isiphelo:

Ukuvavanywa kokuhamba kamva Sesona sixhobo sixhaphakileyo neziqhelekileyo. Nangona kunjalo, kunokubakho imicimbi ngenxa yezizathu ezahlukeneyo umntu anokujongana nazo ngelixa usebenzisa isixhobo. The Isikhokelo seengxaki kwi-assay flow lateral Unceda ukubonisa ukuba kunokwenziwa ntoni ukuhlalutya ingxaki kunye nokusombulula ngakumbi. Ukuqukumbela konke oku kubhaliweyo kungasentla, kunyanzelekile ukuba kuthiwe ukuvelisa ngokukuko ukuvavanywa kokuhamba ngokulandelelana kunye nokuhamba kwemigca yovavanyo lokuhamba, kukho indibaniselwano yemicimbi yebhayoloji, yemichiza, yomzimba neyobunjineli. Kuyacetyiswa kwaye kufuneka kuthathwe amanyathelo okuthatha ingqalelo kuzo zonke izinto ezincinci kunye nemiba ngelixa uvelisa imveliso ukunciphisa naluphi na uhlobo lokwahluka ukuba isixhobo kunye nemicu inokuvelisa iziphumo ezingalunganga ekugqibeleni. Kwakhona, ukuba kukho ingxaki ngenwebu ye-nitrocellulose yesixhobo se-lateral flow assay isixhobo ingxaki inokuba kuyilo lwesixhobo okanye eminye imicimbi yendibano.

Ucaphulo

https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf

https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes

https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting