Ukuhamba ngokuhamba kwexesha kwi-Immnunoassay: I-SWOT yayo

Ukuhamba ngokuhamba kwexesha (Immuno) Uvavanyo; amandla ayo, ubuthathaka, amathuba, kunye nezisongelo

Uvavanyo lwe -lateral flow (Immuno) lolunye lwamaqonga amadala okuxilonga afunyenweyo kwaye kamva aphuhliswa ngeminyaka yoo-1980. Ngokukhula kwetekhnoloji kunye nokuhamba kwexesha, iphaneli yokwahlukahlukana iye yaziswa kwi- Lateral flow (Immuno) Assay . Kuluhlu olubanzi lwee-target analytes, imicu yovavanyo lwe-lateral flow esekelwe kwimigaqo ye-immunochromatographic iyafumaneka. Uvavanyo lokuqala lwenziwe ukuze kuchongwe i-human chorionic gonadotropin (hCG). Njengoko kukhankanyiwe ngasentla ukuba eli qonga lokuxilonga liphenjelelwa yitekhnoloji yanamhlanje, ngoko ke uvavanyo lwe-Lateral Flow (Immuno) luyadityaniswa netekhnoloji kunye nezixhobo ezahlukeneyo ezifana nee-Electrochemical kunye ne-optical transducers kunye nokudluliselwa kwedatha engenazingcingo. Ukuqhubela phambili kunye nokwandiswa kwe-sensitive, multianalyte, kunye ne-quantitative assays eziya kuhlala zikhuphisana kunye nezinye ze-diagnostics ezisetyenziswa kwiilabhoratri zibonisa ukuba ziphambili kwaye ziphucukile.

Uhlalutyo lwe-SWOT lwe- Lateral flow Immuno Assay lunika inkcazo yamandla, ubuthathaka, amathuba, kunye namandla ngokweenkcukacha ezipheleleyo. Okokuqala, sixoxa ngamandla e-Lateral flow Imuuno assay; eyokuqala kukuba isetyenziswa phantse kuyo yonke indawo apho kufuneka khona unyango njengakwiikliniki, amagumbi ongxamiseko, kwigumbi lovavanyo loogqirha, ukuvavanya iikhompawundi ze-organic, iinkqubo zokuxilonga, ukujonga ukubakho okanye ukungabikho kweengozi ezahlukeneyo zosulelo kunye nezifo okanye nakwimizi-mveliso yokutya nokutya, iindawo ze-phytopathological okanye ukungcoliswa kwezinto ezingalunganga zixhaphakile. Kwakhona yindlela yovavanyo ekhethwayo nesetyenziswa kakhulu kwicandelo le-biowarfare, i-forensics, kunye nokuthotyelwa komthetho. Nangona kunjalo, uvavanyo oluzimeleyo lwenziwa kwiisampuli eziziphumo zazo ziphuma zilungile. Kwakhona, kumazwe asakhasayo, i-Lateral Flow Immuno Assay isetyenziswa kakhulu kuba akukho mfuneko yokuba imicu ifakwe efrijini. Ngaphezu koko, ukutolikwa kweziphumo kwanele. I -Lateral Flow Immuno Assays zilahlwa kwaye oku kuthetha ukuba zingasetyenziswa kanye kuphela kwaye kamva zilahlwe.

Ngamandla, ubuthathaka bukhona. Okokuqala, ii-LFA azinakuhlanjwa njengoko zililinge nje inyathelo elinye kwaye ayicetyiswa kakhulu ukuba ibhlokhi ngaphambili. Isizathu kukuba ukuthintela kwangaphambili okanye nokuzihlamba kunokonakalisa ukumanzisa kunye neepropathi zecydrophobicity ezinokubangela amathuba amabi ngakumbi. Obunye ubuthathaka kwiLateral Flow Immuno Assays kukuba ayinakuphathwa kakubi njengoko ikhithi ine-dropper. Ukusetyenziswa kwethropha kuyacokiseka kwaye ukungaziphathi kakuhle kunokubangela iimpazamo kwisiphumo. Ukuza kumthamo wesixhobo esinyukayo isithintelo sinokufumaneka apho. Iivolumu zithintelwe kwi-100 μL kwaye zandiswa ziye kwi-1 milliliter. Ukugqitha kwimida yevolthi kukhokelela ekonakaleni kwe-immuostrip.

Amathuba kwiLateral Flow Immuno Assays aphathelele kwizicelo ezingekho kwilabhoratri. Umzekelo, sibona ukuba uvavanyo lokukhulelwa lwenziwa lula ekhaya. Uvavanyo lwasekhaya luye lwafumana amandla amaninzi kangangokuba lunokwenziwa lula ekhaya kwaye iziphumo zinokufunyanwa. Ngaphezu koko, uvavanyo olungengolwamayeza luyafumaneka olunokufumaneka nge-intanethi okanye kwikhemesti ethile. Amathuba eLateral Flow assay ayanda imihla ngemihla njengoko luyilo lovavanyo oluphezulu kakhulu lokufumana iziphumo ezikhawulezayo njengakwiiwodi zongxamiseko, iikliniki, amapolisa, ukujonga ukungabikho okanye ukubakho kwezifo ezibangelwa ziintsholongwane, uphando lwezonyango, nokunye okuninzi. Elinye ithuba linxulumene nobuthathaka bomthamo obuchazwe ngaphambili. Nangona kukho umda kwimithamo ye-matrix efana negazi, ukubila, i-serum, umchamo, i-plasma, njl. lithuba lokufumana iziphumo ezichanekileyo ngokuhlangabezana novakalelo olufanelekileyo lwe-analyte yangoku. Ukuba i-analyte ekujoliswe kuyo ikhona kwi-matrix yeLateral Flow Immuno Assays (LFAs), umthamo omncinci awunamsebenzi; endaweni yoko, kucetyiswa ngakumbi.

Ngaphandle kwento yokuba iiLateral Flow Immuno Assays zinamandla abo, ubuthathaka, kunye namathuba kukho enye into yohlalutyo lwe-SWOT ekufuneka ingalibaleki kwaye esoyikiso kwi-Lateral Flow Immuno Assays. Njengoko kusaziwa ukuba zininzi iinkqubo zovavanyo olusekwe kubuchwephesha kwaye ibeyinxalenye yolu shishino, ke, izixhobo zekhompyutha kunye nesoftware yezi nkqubo azibizi mali kunokuba zifumaneke lula kwintengiso. Ukuyibonisa ngokukuko ngohlobo lwamagama, kunokubonwa ukuba i-chromatography-mass spectrometry kunye ne-fluid chromatography-mass spectrometry zifuna abasebenzi, kwaye inkqubo yokwenza i-miniaturization yezixhobo kukhokelele ekusetyenzisweni kwabo endaweni yeLateral Flow Immuno Assays. Kwakhona, ifomathi yokuvavanywa kwelabhoratri-kwi-chip iya isiba yinto eqhelekileyo kwaye inokusetyenziswa kunakwisixhobo sokuvavanywa komthamo osecaleni kunye nokuchonga ifomathi. Ngaphaya koko, iimvavanyo ze-enzyme immuno zihlala zenziwa elebhu apho abasebenzi bezobugcisa okanye abasebenzi bakhoyo. Kodwa ngoku sijonga isoyikiso sehardware kunye netekhnoloji iimvavanyo zinokwenziwa ngokusebenzisa izixhobo zokwenza izixhobo ezizenzekelayo ezaziwa ngegama le-ELISA Robots.

isiphelo

Iinkqubo zonyango kumazwe aseNtshona zinokuba negalelo ekunikezelweni kokufakwa kwe-LFA kuyilo lwelebhu-on-a-chip. Ngaphezu koko, ilebhu yeklinikhi ihlala ikufutshane, inika iinkonzo ezongezelelweyo. Iindleko, kwelinye icala, ziya kukhuphuka kakhulu. I-Lateral Flow Immuno Assay yindawo ekhethwayo yezicelo zokwenyani ezingaphandle kwelebhu kwindawo yokhathalelo/imfuneko kwiindawo ezinqongopheleyo zezixhobo. Iistrip zingagcinwa kubushushu begumbi ixesha elide. Oko kuthethiweyo, izinto eziphezulu zohlulo kufuneka zifikeleleke ngokulula, kwaye iziphumo ezibonakalayo zomgangatho ovaliweyo okanye ezilinganayo ezifunyenweyo kusetyenziswa, umzekelo, uyilo lwebhakhowudi okanye i-reflectometer kufuneka zanele. Iifreyimu ezintsha ziye zacetyiswa ukwandisa uvakalelo ngokuqinisa isignali ngee-nanoparticles zegolide ze-colloidal okanye ngokudibanisa ii-nanoparticles zegolide kunye ne-horseradish peroxidase, nto leyo ebangela ukwanda kwesignali. Kukwachazwe indlela yokuxilonga zombini ii-antigens kunye nee-antibodies ngokusebenzisa ii-conjugate pads ezimbini ukuze kumiselwe ubunjani beeproteni ezimbini. Nangona kunjalo, ii-LFA zezona fomathi zovavanyo ezikhethwayo nezisetyenziswa kakhulu zokuxilongwa.

Ucaphulo
https://pubmed.ncbi.nlm.nih.gov/18696055/
https://www.researchgate.net/figure/Advantages-and-disadvantages-of-the-lateral-flow-assay_fig5_304624146
https://link.springer.com/article/10.1007/s00216-008-2287-2
https://viroresearch.com/lateral-flow-assay-advantages-disadvantages/