Ukuvavanywa kokukhulelwa kokuhamba kwexesha kunye nendlela esebenza ngayo?
Ukuhamba kamva Uvavanyo sisixhobo esisetyenziselwa iintlobo ezahlukeneyo zovavanyo ngokufunyanwa komhlalutyi ekujoliswe kuye, ukuba ukhona okanye hayi kwisampulu. Izixhobo zoVavanyo lweeMali ezisecaleni zisetyenziswa kwilizwe jikelele izibonelelo ezininzi kwaye esinye sezibonelelo kukuba zinokusetyenziselwa uvavanyo lwasekhaya. Ngokubhekisele kuvavanyo lwasekhaya, umntu unokuzibona izifo, usulelo, kwaye enze ifayile ye- uvavanyo lokukhulelwa ekhaya. Ukuvavanywa kokuhamba kamva Iimvavanyo zisetyenziselwa ukuchonga i-biomarkers kumchamo, amathe, igazi, iplasma, kunye nezinye iisampuli ngeenjongo zokuxilonga. Ii-LFA eziqhelekileyo zivelisa iziphumo ze-semi-quantitative okanye zekhwalithi, ezifuna ukusetyenziswa kwezixhobo ezikhethekileyo zokufumanisa iziphumo zobuninzi. I-LFA yindlela yokuvavanya esebenzisa iphepha ukufumanisa kunye nokulinganisa abahlalutyi kwimixube enzima. Isampuli ifakwe kwisixhobo sovavanyo, kwaye iziphumo zidla ngokufumaneka kwimizuzu emihlanu ukuya kwengamashumi amathathu.
Izinto ezihamba ngokulandelelana kwezixhobo sisampulu sephedi, iphedi yokudibanisa, inwebu ye-nitrocellulose, kunye nephedi ye-adsorbent. Xa isampulu okanye imatriki ifakwa kwisampulu yephedi iqukuqela isiya kwiphedi edityanisiweyo apho iantigens zebhayoloji kunye nezibulala-ntsholongwane ziqala ukubopha kunye namasuntswana avela kumxube wesampulu yepad kunye neeanoparticles kwiphedi edibeneyo. Emva koko, baya kwi-membrane. Apha ii-nanoparticles ziya kuba ngumhlalutyi ziboshwe kwaye kwisixhobo soVavanyo soVavanyo lweLateral imigca emibini ilayini yovavanyo kunye nomgca wolawulo. Ezi nanoparticles ziya kuqala ukuhamba ngapha nangapha komgca wovavanyo okanye kumgca wokulawula.
Umgca wovavanyo uqukethe ii-nanoparticles ezingafakwanga ezinokudibanisa namasuntswana kwiphedi edibeneyo ukubonisa ubukho bohlalutyo ekujoliswe kulo. Nangona kunjalo, ukusuka kumgca wovavanyo, ulwelo ludlulela kumgca wolawulo. Umgca wolawulo uqulethe iigundand zobudlelane ezisebenza ukubopha amasuntswana okudibanisa kunye nohlalutyo okanye ngaphandle komhlalutyi ukubonisa ukuba isixhobo se-assay esisecaleni sisebenza kakuhle kwaye ngokufanelekileyo. Ngoku, emva kokuba ulwelo ludlulile ukusuka kwimigca yomibini okt umgca wovavanyo kunye nomgca wolawulo kukho iphedi yentengiso. Iphedi yeAdsorbent isebenza kwintengiso yazo zonke izinto ezingamanzi ezingaphezulu. Kuba ixabiso elincinci nelilawulwayo le-matrix liyafuneka, ke ngoko, liqinisekisa ukuvumela ukuhamba kolwelo olufunekayo kuphela.
Ngelixa usenza iimvavanyo zokukhulelwa kwi izixhobo flow lateral Ezi ziifomathi ezimbini, oko kukuthi, ifomathi yokhuphiswano kunye nefomathi yesandwich. Ukusetyenziswa kwefomathi yokhuphiswano kukufumanisa ii-analytes xa ii-antibodies okanye i-analyte ayanelanga kwi-multiple binding. Kwifomathi yokhuphiswano, kukho ii-molecules ze-analyte kumgca wovavanyo kunye ne-protein-analyte complex ene-conjugate pad echaza ukuba kukho okanye akukho i-analyte. Kolu hlobo lwefomathi, ukuba i-target analyte ayikho, ii-conjugates ziya kunamathela kumgca wovavanyo. Ukufika kwifomathi yesandwich yovavanyo lokukhulelwa lwezixhobo ze-lateral flow assay, le fomathi isetyenziselwa ukufumanisa ii-target analytes, ukuba zikhona okanye azikho. Ngaphezu koko, ezi analytes zinkulu kufuneka zibe nee-epitopes. Kwakhona ii-antibodies kwiindawo ezimbini zokubhida zale fomathi zibopha ngendlela enye i-analyte eya kubopha ngayo kunye nee-conjugated particles kunye nenye i-analyte enolunye uvavanyo lokubopha oluya kusetyenziswa kumgca wovavanyo lwe-lateral flow Assay. Nangona kunjalo, ukuba i-target analyte ifunyenwe iya kubopha kunye ne-antibody-nanoparticle conjugate kwaye ivelise iziphumo ezifunwayo. Kwakhona, ingongoma ebalulekileyo kukuba kwifomathi yesandwich yesixhobo se-latera Flow essay sovavanyo lokukhulelwa, umgca onemibala uvela oko kuthetha ukuba iziphumo zilungile. Nangona kunjalo, kwifomathi yokhuphiswano kuya kubakho ukungabikho/ukunyamalala kwemigca enemibala kwisixhobo oko kuthetha ukuba iziphumo zilungile. kwisixhobo sovavanyo lwe-lateral flow assay sisebenza ngale ndlela yokwenza uvavanyo lokukhulelwa kuso.
Kuhlalutyo lovavanyo lokuvavanywa kwecala lokuhamba, kubalulekile ukuba uqaphele ukuba iimvavanyo ziya kuba kwimiqondiso enokufundwa ngamehlo kwaye iyaqondakala ngokulula. Ukuqinisekisa ubuntununtunu besixhobo esisecaleni sokungena kwesixhobo kubalulekile ukuba ube nophawu lomeleleyo olunokwenzeka ukuya kuthi ga kwinqanaba. Ukuba kukho amasuntswana amakhulu ukubopha kuya komelela kodwa ukuba kuya kuba kukhulu kakhulu kuyakubakho isithintelo ekuhambeni kwesampulu. Iziphumo zesixhobo sokuvavanywa kokuhamba ngokulandelelana kuya kuba kwezi fomathi zilandelayo okt umgangatho, umgangatho ophakathi, kunye nobungakanani. Nangona kunjalo, iimvavanyo zokukhulelwa kwisixhobo se-assay flow lateral, ziya kubonisa iziphumo ezisemgangathweni, okt Ewe okanye Hayi Ukuvavanywa kokukhulelwa kokuhamba kwexesha, uhlalutyo kunye neziphumo zisekwe kwi HCG Uhlalutyo. I-HCG imele i-chorionic gonadotropin yabantu eyenziwe ziiseli ze-trophoblast. Xa i-matrix ifakwa kwisampulu yesixhobo, i-chorionic gonadotropin ibotshelelwe kwii-nanoparticles zegolide ezidityaniswe ne-antibody yovavanyo kunye ne-anti-conjugated antibody. Kuba oku kukudityaniswa okuqhelekileyo kwe-hCG kumchamo wakho, ukugxila kwayo kuyahluka ngokuxhomekeka kuvavanyo lwakho. Umda wokufunyanwa kovavanyo uhlala uphakathi kwe-20 kunye ne-100 mIU / mL. Ukuthetha malunga noxilongo lobungakanani, ukunyanzeliswa komgca wovavanyo kumiselwe ngumgangatho osetiweyo okt ubizwa ngokuba ngumgangatho wokulinganisa kwaye koku, ii-analytic ziguqulwa zaba lixabiso loxinzelelo. Ukumisela iziphumo ezichanekileyo, kucetyiswa ukuba kusetyenziswe umfundi wokufunda ukuze afunde iziphumo kwisixhobo sovavanyo soVavanyo lweLateral.
isiphelo
Izixhobo zoVavanyo lweeMali ezisecaleni zibiza indleko kwaye kulula ukuzisebenzisa izixhobo ezisetyenziswa ngamazwe ahambele phambili kunye nasaphuhlayo. Ngaphaya koko, izixhobo zokuhamba ngokulandelelana yenye yeendlela zokuvavanya ezinokuthenjwa iimvavanyo zokukhulelwa njengoko zivelisa iziphumo eziyinyani kwaye phantse wonke umntu uyazi ukuba angazisebenzisa njani xa ziluncedo. Isixhobo sovavanyo lweLateral Flow sesona sona siphezulu sokwenza naluphi na uhlobo lovavanyo ekhaya.
Ucaphulo
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4