Indlela yokuvavanywa kweMali yokuGqibela kweNyathelo ngenyathelo ngenyathelo

Ukuhamba kamva IAsay, isixhobo okanye imicu yovavanyo esetyenziselwa ukumisela ubukho okanye ukungabikho komhlalutyi kwisampulu, ineendlela ezilandelwa kuyo yonke inkqubo yovavanyo. Inkqubo iqinisekisa ukusebenza kovavanyo, ukwenza inkqubo, kunye nokufumana iziphumo zovavanyo. Ukongeza inyani, kufanele kuqatshelwe ukuba kwane teknoloji ibikhona kuyo yonke indawo kwezonyango kwaye Ukuvavanywa kokuhamba kamva ahlukanga kuloo nto. Ke ngoko, itekhnoloji ibandakanyiwe kwifayile ye- Ukuvavanywa kokuhamba kamva kodwa ukusebenza kwabo okanye indlela yokusebenza kuhlala injalo. Kwaye esi sesinye sezizathu sesona sixhobo siphambili sokuvavanya kumazwe athuthukileyo nasaphuhlayo.

Ukuqonda indlela esebenza ngayo Ukuvavanywa kokuhamba kamva, nalu ushwankathelo olufutshane loomatshini bayo. Okokuqala, kukho isampulu pad apho kulayishwa khona isampulu, kwaye eqhubeka nokuya kwiphedi yokudibanisa. Kwipali yokudibanisa, iziganeko ezibophayo zenzeka ngenxa yobukho bezinto ezinokubakho ezinokuthi zibe zegolide okanye i-latex. Inyathelo elilandelayo elenzekayo kukuba isampulu kunye nearhente yokuphendula iyabopha kwaye ihambele kwimbonakalo yempendulo. Inwebu yokuphendula inceda ekuveliseni iziphumo. Umgca wovavanyo uyayeka okanye uqhubeke nokuhamba de ipad adsorbent. Ukwazi ukuba i-Lateral Flow Assay isebenze iiarhente zokuphendula zibopha kulayini wokulawula kwaye zivelise iziphumo. Inkqubo eneenkcukacha yoVavanyo lokuHamba kweThagethi ichazwe ngokulandelayo.

Isampulu yePad

Isampulu yePad sisisiqalo sovavanyo lokuvavanywa kokuhamba ngokulandelelana. Ukwenziwa kwephedi yesampulu yesampulu yiglasi yefayibha, imisonto ephothiweyo, okanye iselulosi. Ifayibha yeglasi, umnatha olukiweyo, kunye neselulosi ezizezazo iipropathi ezahlukeneyo. Ukusebenza kunye neendleko zazo zixhomekeke kuhlobo. Ngokusisiseko, isampulu yesicelo sephedi iqala kuvavanyo lokuvavanywa kokuhamba ngokuhamba kwexesha kunye nomsebenzi ophambili woku kukufumana ubungakanani besampuli okanye isampulu kwaye uyihambise. Kubalulekile ukuba isampulu iphedi ivumele ukutyibilika kwesampulu ukuze ikwazi ukwahlula isampulu, uhlengahlengiso lwe-pH, kunye nokubopha ngokulinganayo. Ukuze uvavanyo luqaliswe, isampuli kufuneka ibekwe kwisampulu yesicelo sephedi.

Ukudibanisa iPad

I-conjugate pad linyathelo lesibini lendlela yovavanyo lweLateral Flow. I-Conjugate Release Pad inefayibha yeglasi engalukwanga kwaye kukho i-ejenti yokufumanisa esele yomile. Ukuyidibanisa ne-sample application pad, xa isampuli ifikelela kwinqanaba lokugcwala kwaye igeleza kwi-conjugate release pad kwaye apha i-ejenti yokufumanisa ikhutshwa ukuze ihlalutye isampuli. Umsebenzi ophambili we-conjugate pad kukuqinisekisa ukuba isampuli kunye ne-reaction agent zifuduselwa kwi-membrane ngelixa zigcina i-conjugate. Kwi-conjugate pad, kufuneka kubekho i-non-specific binding ephantsi ukuqinisekisa ukuba i-detection reagent ayibambeki kwi-pad.

Ukufunyanwa kweReagent

Kuhlala kukho ukubonwa kwakhona kwe-reagent kwi Uvavanyo lokuvavanywa kokuhamba kamva. I-reagent reagent ine-antibodies ezidityaniswa ngakumbi ne-fluorescent okanye amasuntswana anemibala. Ukusebenza kwe-assay yokuhamba kwecala kuxhomekeke kumgangatho we-antibodies kunye ne-reagent reagent.

Umntu ongahlaseli

Ukukhethwa kwe-antibody kuvavanyo lokuvavanywa kokuhamba komva kuthathwa njengokubalulekileyo kwaye izinto ezimbalwa kufuneka ziqwalaselwe ngelixa kukhethwa i-antibody ye-lateral flow assay. Into yokuqala kukubonelelwa okungcono kwe-antibody. Okwesibini, iinkcukacha ze-antigen kufuneka zihlolwe kwaye ziqinisekiswe. Kwakhona, ukhetho phakathi kwe-monoclonals okanye i-polyclonals xa usenza ukhetho kwii-antibodies. IiMonoclonals ziyacetyiswa ngenxa yokuba kunokubakho ukuveliswa kwenani elikhulu lamachiza omzimba kwaye ke oko kuyanceda ekuchongeni i-antigenic epitope enye ngelixa iiPolycilonals zinokuba yinto exhomekeke kwinto eyahlukileyo kwaye unikezelo lwayo aluqinisekiswanga nokuba loluphi na.

Inwebu yeNitcocellulose

I-Substrate Membrane okanye iNitcocellulose Membrane yenye yezona zinto zibaluleke kakhulu nezibalulekileyo ekumiseleni i-Lateral Flow Assay kunye nokuqonda kwayo. Umgca wovavanyo kunye nomgca wokulawula utsaliwe ngaphezulu kwenwebu ukubonelela ngokufanelekileyo ekubambeni ii-antibodies. I-adsorption engachazwanga kwimigca yovavanyo kunye nakwimigca yolawulo inokuchaphazela ubuntununtunu beziphumo ezinokuvelisa iziphumo ezingezizo.

Iphedi yeAdsorbent

I-adsorbent Pad okanye i-Wicking pad ibekwe ekupheleni komcu okanye isixhobo sokuvavanywa komva wokuhamba. Indawo ekubekwe kuyo iphedi yokujija inokuba nefilitha yeselulose ethi helios ekusebenziseni ubungakanani besampulu kumtya wovavanyo. Owona msebenzi uphambili we-adsorbent pad kukulawula ukuhamba kwesampulu yolwelo ngaphezulu kwenwebu ye-nitrocellulose. Ayisiyiyo loo nto kuphela, kodwa ikuyekisa nokubuyela umva kwesampulu ngokugcina ubungakanani oboneleyo besampulu okanye isampulu.

Inkqubo yoKhetho kwiNkqubo yoVavanyo loVavanyo lweLateral

Nokuba inyathelo ngenyathelo ngenyathelo okanye indlela yokusebenza ichaziwe ngokweenkcukacha, ukhetho lwenkqubo kwi-assal flow flow lubalulekile kuyo. Zimbini iindlela onokukhetha kuzo; enye kukuqhubekeka kwebatch kwaye enye ikwi-line / reel-to-reel Assembly. Ukulungiswa kweBatch kusebenza ngokukuko kwaye kudinga abasebenzi abanokukhokelela ekubeni umahluko kwiziphumo, ngaloo ndlela kuveliswe iziphumo ezingathembekiyo. Ngelixa i-reel-to-reel Assembly ingumatshini olungiselelwe ukwenziwa kunye nokuthenjwa ngakumbi kumanani amakhulu okanye amaninzi. Kuzo zombini ezi ntlobo zokukhetha ukusebenza, umba wobushushu ubaluleke kakhulu kwaye kufuneka uthathelwe ingqalelo ukuvelisa iziphumo eziyinyani nezinqwenelekayo.

isiphelo

The Indlela yokuvavanywa kweMali yokuThamba iquka iindawo ezimbalwa ezibandakanya iipads, inwebu, kunye namanye amasuntswana anceda ukufumana isampulu kunye nokuvelisa iziphumo. Nangona kukho izixhobo ezahlukeneyo zoVavanyo lokuHamba kweLateral kwaye ezinye zazo zibandakanyiwe kubuchwephesha ngelixa ezinye zingenjalo kodwa indlela yokusebenza ihlala ifana. Zonke izinto ezisebenza ngendlela yoVavanyo lokuHamba kweXesha eliseMva kufuneka zithathelwe ingqalelo njengezibalulekileyo kwaye akufuneki zihoyiwe.

Ucaphulo
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test