Indlela yokuvavanywa kweMali yokuGqibela kweNyathelo ngenyathelo ngenyathelo

Uvavanyo lweLateral Flow , uvavanyo lwesixhobo okanye imicu olusetyenziselwa ukufumanisa ukuba kukho okanye akukho mhlalutyi kwisampulu, lunendlela elandelwayo kuyo yonke inkqubo yovavanyo. Le ndlela iqinisekisa ukusebenza kovavanyo, ukuqhuba inkqubo, kunye nokufumana iziphumo zovavanyo. Ukongeza inyani, kufuneka kuqatshelwe ukuba netekhnoloji ibikho kuyo yonke indawo kwicandelo lezonyango kwaye iiLateral Flow Assays azahlulwanga kuloo nto. Ke ngoko, le teknoloji ifakiwe kwiLateral Flow Assay kodwa indlela esebenza ngayo okanye indlela esebenza ngayo ihlala ifana. Kwaye le yenye yezizathu zokuba sisixhobo sovavanyo esiphambili kumazwe aphuhlileyo nasaphuhlayo.

Ukuze uqonde indlela esebenza ngayo iLateral Flow Assay , nantsi inkcazelo emfutshane ngendlela esebenza ngayo. Okokuqala, kukho isampuli yephedi apho isampuli ilayishwa khona, kwaye ihambela phambili kwi-conjugate pad. Kwi-conjugate pad, iziganeko zokubopha zenzeka ngenxa yokubakho kwamasuntswana okufumanisa anokuba yigolide okanye i-latex. Inyathelo elilandelayo elenzekayo kukuba isampuli kunye ne-reaction agent zibopha kwaye zihambe ziye kwi-reaction membrane. I-reaction membrane inceda ekuveliseni iziphumo. Umgca wovavanyo uyama okanye uqhubeke ugeleza de kube yi-adsorbent pad. Ukuze wazi ukuba i-Lateral Flow Assay iyasebenza na, ii-reaction agents zibopha kumgca wolawulo kwaye zivelise iziphumo. Indlela eneenkcukacha yeLateral Flow Assay ichazwe ngokulandelayo.

Isampulu yePad

Isampulu yePad sisisiqalo sovavanyo lokuvavanywa kokuhamba ngokulandelelana. Ukwenziwa kwephedi yesampulu yesampulu yiglasi yefayibha, imisonto ephothiweyo, okanye iselulosi. Ifayibha yeglasi, umnatha olukiweyo, kunye neselulosi ezizezazo iipropathi ezahlukeneyo. Ukusebenza kunye neendleko zazo zixhomekeke kuhlobo. Ngokusisiseko, isampulu yesicelo sephedi iqala kuvavanyo lokuvavanywa kokuhamba ngokuhamba kwexesha kunye nomsebenzi ophambili woku kukufumana ubungakanani besampuli okanye isampulu kwaye uyihambise. Kubalulekile ukuba isampulu iphedi ivumele ukutyibilika kwesampulu ukuze ikwazi ukwahlula isampulu, uhlengahlengiso lwe-pH, kunye nokubopha ngokulinganayo. Ukuze uvavanyo luqaliswe, isampuli kufuneka ibekwe kwisampulu yesicelo sephedi.

Ukudibanisa iPad

I-conjugate pad linyathelo lesibini lendlela yovavanyo lweLateral Flow. I-Conjugate Release Pad inefayibha yeglasi engalukwanga kwaye kukho i-ejenti yokufumanisa esele yomile. Ukuyidibanisa ne-sample application pad, xa isampuli ifikelela kwinqanaba lokugcwala kwaye igeleza kwi-conjugate release pad kwaye apha i-ejenti yokufumanisa ikhutshwa ukuze ihlalutye isampuli. Umsebenzi ophambili we-conjugate pad kukuqinisekisa ukuba isampuli kunye ne-reaction agent zifuduselwa kwi-membrane ngelixa zigcina i-conjugate. Kwi-conjugate pad, kufuneka kubekho i-non-specific binding ephantsi ukuqinisekisa ukuba i-detection reagent ayibambeki kwi-pad.

Ukufunyanwa kweReagent

Kusoloko kukho i-reagent yokufumanisa kuvavanyo lokuhamba kwe-lateral flow . I-reagent yokufumanisa ine-antibodies ezidityaniswe ngakumbi ne-fluorescent okanye amasuntswana anemibala. Ukusebenza kovavanyo lokuhamba kwe-lateral kuxhomekeka kumgangatho we-antibodies kunye ne-reagent yokufumanisa.

Umntu ongahlaseli

Ukukhethwa kwe-antibody kuvavanyo lokuvavanywa kokuhamba komva kuthathwa njengokubalulekileyo kwaye izinto ezimbalwa kufuneka ziqwalaselwe ngelixa kukhethwa i-antibody ye-lateral flow assay. Into yokuqala kukubonelelwa okungcono kwe-antibody. Okwesibini, iinkcukacha ze-antigen kufuneka zihlolwe kwaye ziqinisekiswe. Kwakhona, ukhetho phakathi kwe-monoclonals okanye i-polyclonals xa usenza ukhetho kwii-antibodies. IiMonoclonals ziyacetyiswa ngenxa yokuba kunokubakho ukuveliswa kwenani elikhulu lamachiza omzimba kwaye ke oko kuyanceda ekuchongeni i-antigenic epitope enye ngelixa iiPolycilonals zinokuba yinto exhomekeke kwinto eyahlukileyo kwaye unikezelo lwayo aluqinisekiswanga nokuba loluphi na.

Inwebu yeNitcocellulose

I-Substrate Membrane okanye iNitcocellulose Membrane yenye yezona zinto zibaluleke kakhulu nezibalulekileyo ekumiseleni i-Lateral Flow Assay kunye nokuqonda kwayo. Umgca wovavanyo kunye nomgca wokulawula utsaliwe ngaphezulu kwenwebu ukubonelela ngokufanelekileyo ekubambeni ii-antibodies. I-adsorption engachazwanga kwimigca yovavanyo kunye nakwimigca yolawulo inokuchaphazela ubuntununtunu beziphumo ezinokuvelisa iziphumo ezingezizo.

Iphedi yeAdsorbent

I-adsorbent Pad okanye i-Wicking pad ibekwe ekupheleni komcu okanye isixhobo sokuvavanywa komva wokuhamba. Indawo ekubekwe kuyo iphedi yokujija inokuba nefilitha yeselulose ethi helios ekusebenziseni ubungakanani besampulu kumtya wovavanyo. Owona msebenzi uphambili we-adsorbent pad kukulawula ukuhamba kwesampulu yolwelo ngaphezulu kwenwebu ye-nitrocellulose. Ayisiyiyo loo nto kuphela, kodwa ikuyekisa nokubuyela umva kwesampulu ngokugcina ubungakanani oboneleyo besampulu okanye isampulu.

Inkqubo yoKhetho kwiNkqubo yoVavanyo loVavanyo lweLateral

Nokuba inyathelo ngenyathelo ngenyathelo okanye indlela yokusebenza ichaziwe ngokweenkcukacha, ukhetho lwenkqubo kwi-assal flow flow lubalulekile kuyo. Zimbini iindlela onokukhetha kuzo; enye kukuqhubekeka kwebatch kwaye enye ikwi-line / reel-to-reel Assembly. Ukulungiswa kweBatch kusebenza ngokukuko kwaye kudinga abasebenzi abanokukhokelela ekubeni umahluko kwiziphumo, ngaloo ndlela kuveliswe iziphumo ezingathembekiyo. Ngelixa i-reel-to-reel Assembly ingumatshini olungiselelwe ukwenziwa kunye nokuthenjwa ngakumbi kumanani amakhulu okanye amaninzi. Kuzo zombini ezi ntlobo zokukhetha ukusebenza, umba wobushushu ubaluleke kakhulu kwaye kufuneka uthathelwe ingqalelo ukuvelisa iziphumo eziyinyani nezinqwenelekayo.

isiphelo

I -Lateral Flow Assay Mechanism inamacandelo ambalwa aquka ii-pads, ii-membranes, kunye namaqhekeza athile anceda ekufumaneni isampuli kwaye avelise iziphumo. Nangona kukho izixhobo ezahlukeneyo ze-Lateral Flow Assay kwaye ezinye zazo zifakwe ngobuchwepheshe ngelixa ezinye zingenazo, kodwa indlela esebenza ngayo ihlala ifana. Zonke izinto ezikwindlela yokusebenza kwe-Lateral Flow Assay kufuneka zithathwe njengezibalulekileyo kwaye akufuneki zijongwe.

Ucaphulo
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test