ISIBANGELA - I-Latarl Flow Assay Materials & Izinsiza kusebenza ze-lateral Flow Production
ukuthuthukiswa kwe-lateral

Ukuhlolwa kokugeleza kokuhamba kwesikhathi: Izimiso, Imiklamo, namalebula

Posted on August 28, 2021 by Lisa 

Ukugeleza Kwangemuva Izinsizakalo amadivayisi asetshenziselwa ukwenza izinhlobo ezahlukahlukene zokuhlolwa nephuzu lokuhlolwa kokunakekelwa futhi kwaziwa nangokuthi ukuhlolwa kwangempela. Ama-Lateral Flow Assays amadivayisi amancane futhi amancane asetshenziswa cishe kuyo yonke imikhakha yezokwelapha lapho kutholakala khona ama-analyties. Uma ubheka isimo samanje emhlabeni jikelele, zisetshenziselwa nokuhlola iCoronavirus. Njengoba zinokwethenjelwa, zingambi eqolo, futhi zifinyeleleka kuwo wonke umuntu esetshenziselwa yona ukuhlolwa kokukhulelwa, ukutheleleka kwamagciwane, ukutheleleka ngegciwane, kanye nokutheleleka kwezifo. Ukuhlolwa kwe-Lateral flow Assays akudingi noma yiluphi uhlobo lwabasebenzi abaqeqeshiwe noma abakhethekile. I- ukulingana kokugeleza kwe-lateral inqubo isebenza ekuhlanganisweni kwamakhemikhali omzimba nama-antigen noma i-DNA target- i-DNA hybridization.

Izimiso

Izimiso lapho i- Ukuhlanjululwa Kwesikhathi Esizayo imisebenzi iyisisekelo impela. Ku-Lateral Flow Assay, isampula ketshezi noma i-matrix igeleza ngosizo lwamandla angunaphakade okusho isenzo se-capillary. Umatikuletsheni unomhlaziyi ohlosiwe odingekayo ekukhiqizeni imiphumela yesikhangiso. Uketshezi lugeleza ngemichilo ehlukahlukene yama-polymeric lapho ama-analytic nama-molecule angahlanganiswa khona. Umgomo wonke usebenza ezingxenyeni ezahlukahlukene ze-Lateral Flow assay njengoba kunamaphakethe ahlukene lapho uketshezi kufanele lugeleze khona ukukhiqiza imiphumela. Isinyathelo sokuqala sokusebenzisa le divayisi ukuthi isampula lifakwa kudivayisi egelezelwa kudivayisi ye-lateral flow ngokugcwele. Uma igeleza inqubo yokuhlunga yenzeka lapho izinhlayiya ezingafuneki noma ezingathandeki zingasuswa noma zihlungwe. Ngaphakathi kwesikhathi esinikeziwe sokuqedwa kokuhlolwa kokuhlolwa kwe-lateral flow, yonke inqubo yenzeka ngakho-ke imiphumela iyabonakala. Lapho isampula lifinyelela kuphedi yokuhlangana yedivayisi izinhlayiya ezomisiwe kuphedi ziyaxubana nezinhlayiya zesampula. Isinyathelo esilandelayo esenzekayo ukugeleza kwama-nanoparticles aboshwe yi-analyte nokuthola ukufinyelela kolayini bokulawula okuqhubeka kuholele emiphumeleni. I-specimen ingaqala ukugeleza kuphedi ye-conjugate, equkethe ama-nanoparticles anemibala kakhulu noma i-fluorescent ene-antibody kusendlalelo sayo esiphezulu. Kukhona nephedi yokukhotha ebamba isampula noma utshwala be-specimen ngokuphelele ukuqinisekisa ukuthi alukho uketshezi olushiywe ngemuva kolayini bokuhlola nokulawula. Ngemuva kokuthi inqubo iqede ngokulandela umthetho wayo wokusebenza imiphumela iyatholakala.

othomathikhi-inhlangano-umshini
othomathikhi-inhlangano-umshini

Designs

Imiklamo ye- ukugeleza kwe-lateral i-assay yakhiwa uhlelo lwe-chromatographic. Kuhlelo lwe-chromatography, izingxenye zihlukaniswa ngokuya ngokuhamba kwazo ngokusebenzisa ulwelwesi lokuphendula kanye nokuphendula komzimba njengokuphendula kwe-antigen ne-antibody noma i-DNA hybridization, kanye ne-nucleic-acid target analyte. Ngaphezu kwalokho, i-lateral flow Assay yakhelwe nge-pad yesampula, i-conjugate bad, i-nitrocellulose ulwelwesi, amalebula, ama-nanoparticles, i-adsorbent pad wicking, ne-buffer enamathiselwe kuyo kwamanye amadivayisi kuphela. Imiklamo yokuhlolwa kwe-lateral flow kuncike ekusetshenzisweni, ekusimeni, ekusebenzeni nasekuphepheni. Kunamamojula okufanele alandelwe ekwakhiweni kwezilingo zokugeleza kwe-lateral namadivayisi wokuhlola. Kumiklamo ye-lateral flow assay, izidingo zomzimba zibaluleke kakhulu. Lezi zidingo zomzimba ze-LFA yizilinganiso ze-cartridge, izici ze-cartridge okusho ukuthi i-conjugate pad ne-sample pad, imininingwane yabafundi, njll. Ukwakheka kwe-LFA nakho kuncike ekusebenzeni nasezimpawu zedizayini ethile. Isibonelo, ukusebenza nezici ezihlobene nakho kuzofaka indlela yokuhlaziya, ifomethi yokuhlaziya okuqondiwe kokuhlola, ukusebenza komtholampilo, ukuzwela nomkhawulo wokucaciswa, njalonjalo. Ezinye izinto ezimbili ezibalulekile ekwakhiweni kwe-lateral flow assay zisingatha nezidingo zokuphotha. Ukuphathwa kwezidingo zokwakhiwa kwe-LFA kuhambisana kakhulu nemvelo, nempilo yegobolondo. Ngenkathi izidingo zesampula zedizayini yokuhlolwa kwe-lateral flow assay kuyizinhlobo zesampula, izinga lokushisa lapho isampula lingagcinwa khona, izinga lokushisa elidingekayo ekugcineni, kanye nokwelashwa kwesampula.

ikhithi yokuthuthukiswa kwendawo yonke
ikhithi yokuthuthukiswa kwendawo yonke

Amalebula

Kwi-Lateral Flow Assay, kunezinto ezahlukahlukene ezisetshenzisiwe ezinikezwa igama lamalebula. Amanye amalebula asetshenziswa ku-LFA ngama-nanoparticles egolide, amachashazi e-quantum, ubuhlalu be-latex enemibala, ama-nanoparticles esiliva, i-selenium nanoparticles, ama-enzyme, i-liposomes, namanye amaningi. Nawa amalebula ambalwa ku-LFA okuzoxoxwa ngawo. Noma yiliphi ilebula elisetshenziswa ku-Lateral Flow Assay kufanele litholakale endaweni ephansi kakhulu futhi izakhiwo zamalebula kufanele zigcinwe kuze kuhlangane nama-molecule we-biorecognition. Okokuqala, ama-nanoparticles egolide we-colloidal angenye yamalebula avame kakhulu. Izinhlayiya zegolide zeColloidal azinamandla futhi zinokuma okuhle kwesigaba. Ngaphezu kwalokho, ama-nanoparticles egolide e-colloidal anokuhlangana okuphezulu maqondana nama-biomolecule okwenza ukusebenza kwawo kube lula. Ubukhulu nokuma kwazo kuyahlukahluka. Ezinye zezici ezivelele kakhulu zama-nanoparticles egolide ukwakheka kwazo okulungele imvelo, ukuhlangana kwazo okuphezulu maqondana nama-molecule namaprotheni, ukusayinda okuhle kwe-optical, nokuzinza kwazo. Enye ilebula esetshenziswa kuma-LFAs yizinhlayiya kazibuthe nezihlanganisi ezisetshenziselwa ukusayina kumadivayisi. Izinhlayiya ezinemibala kazibuthe ku-LFA ziyasetshenziswa noma zikhiqiza imigqa enombala efundwa yinsimbi noma isifundi sokuqhafaza. Kukhona nesifundi se-magnetic assay esisetshenziselwa ukufunda amasiginali avela kubo. Ama-enzyme nawo asetshenziswa ku-LFA futhi andisa isinyathelo esisodwa kuyo yonke inqubo. Ingeza isinyathelo sokungeza i-substrate efanelekile ngemuva kokuqedwa kwesilingo. I-colloidal carbon nayo isetshenziswa kuma-LFA kanye nombala omnyama. Ngenxa yombala wazo, zitholakala kalula ngokuzwela okuphezulu.

ishidi lokuhlola okusheshayo-elingasikiwe
ishidi lokuhlola okusheshayo-elingasikiwe

Isiphetho

The Ukuhlolwa kwe-Lateral Flow Assay zingenye zamadivayisi asetshenziswa kakhulu. Izimiso, ukwakheka, namalebula we-lateral flow assay somandla onke kwehlukile kwamanye amadivayisi we-Lateral Flow Assay nawo aguqula ukusebenza kwaleyo divayisi ethile ye-lateral flow assay. Yonke imigomo, ukwakheka namalebula kuyizinto ezibalulekile zama-LFA.

Okubhekwayo

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4986465/

https://nanocomposix.com/pages/lateral-flow-assay-design

https://www.nature.com/articles/s41598-018-35694-9

https://www.sciencedirect.com/science/article/pii/S131961031400129X

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