I-lateral Flow Assay Mechanism of Action Isinyathelo ngesinyathelo
I-Lateral Flow Assay , ukuhlolwa kwedivayisi noma ama-strips okusetshenziselwa ukuthola ukuthi kukhona noma akukho yini i-analyte kusampula, inohlelo olulandelwayo kuyo yonke inqubo yokuhlola. Lolu hlelo luqinisekisa ukusebenza kokuhlolwa, ukwenza inqubo, nokuthola imiphumela yokuhlolwa. Ukwengeza iqiniso, kufanele kuqashelwe ukuthi ngisho nobuchwepheshe bukhona yonke indawo emkhakheni wezokwelapha futhi i-Lateral Flow Assays ayihlukanisiwe nalokho. Ngakho-ke, ubuchwepheshe bufakwe ku- Lateral Flow Assay kodwa ukusebenza noma indlela yabo ihlala ifana. Futhi lesi ngesinye sezizathu ezenza kube yithuluzi lokuhlola elihamba phambili emazweni athuthukile nasathuthuka.
Ukuze uqonde indlela esebenza ngayo i- Lateral Flow Assay , nansi incazelo emfushane ngendlela esebenza ngayo. Okokuqala, kukhona iphedi yesampula lapho isampula ilayishwa khona, futhi eqhubeka isiya ephedi ye-conjugate. Ephedi ye-conjugate, izehlakalo zokubopha zenzeka ngenxa yokuba khona kwezinhlayiya zokuthola ezingaba yigolide noma i-latex. Isinyathelo esilandelayo esenzeka ukuthi isampula kanye ne-reaction agent zibophe futhi zithuthele ephedi ye-reaction. I-reaction membrane iyasiza ekukhiqizeni imiphumela. Umugqa wokuhlola uyama noma uyaqhubeka nokugeleza kuze kube yiphedi ye-adsorbent. Ukuze wazi ukuthi i-Lateral Flow Assay iyasebenza yini, ama-reaction agents ayabopha emgqeni wokulawula futhi akhiqize imiphumela. Indlela eningiliziwe ye-Lateral Flow Assay ichazwe ngokulandelayo.
Isampuli Pad
Isampula Pad ukuqala kokuhlolwa kwe-assay flow lateral. Ukwakheka kwephedi yokufaka isampula yi-fiber fiber, i-mesh ephothiwe, noma i-cellulose. I-fiber fiber, i-mesh ephothiwe, ne-cellulose inezakhiwo ezihlukile komunye nomunye. Futhi, ukusebenza nezindleko zazo futhi kuya ngohlobo. Ngokuyisisekelo, iphedi lesampula lesampula liqala ukuhlolwa kwe-Lateral Flow Assay futhi umsebenzi oyinhloko walokhu ukuthola ubuningi besifanekiso noma isampula bese usabalalisa. Kubalulekile ukuthi isampula pad livumele bushelelezi besampula ukuze ikwazi ukwehlukanisa isampula, ukulungiswa kwe-pH, nokubopha ngokulinganayo. Ukuze isivivinyo siqaliswe, isampula kufanele lifakwe kuphedi yohlelo lokusebenza lwesampula.
Isihlanganisi Pad
Iphedi ye-conjugate iyisinyathelo sesibili sendlela yokulinganisa i-Lateral Flow Assay. Iphedi Yokukhulula I-Conjugate yakhiwe nge-fiber yengilazi engalukiwe futhi kukhona i-ejenti yokuthola eseyomile kakade. Ukuyixhumanisa nephedi yokufaka isampula, lapho isampula ifika endaweni yokugcwala futhi igeleza iye kuphedi yokukhulula i-conjugate futhi lapha i-ejenti yokuthola ikhishwa ukuze ihlaziye isampula. Umsebenzi oyinhloko wephedi ye-conjugate ukuqinisekisa ukuthi isampula kanye ne-ejenti yokusabela kuthuthelwa ku-membrane ngenkathi kugcinwa ne-conjugate. Kuphedi ye-conjugate, kumele kube nokubopha okuphansi okungaqondile ukuqinisekisa ukuthi i-reagent yokuthola ayinamathele kuphedi.
Ukutholwa kwe-Reagent
Kuhlala kukhona i-reagent yokuthola ekuhlolweni kokugeleza kwe-lateral . I-reagent yokuthola inama-antibodies ahlanganiswe ne-fluorescent noma izinhlayiya ezinemibala. Ukusebenza kahle kwe-lateral flow assay kuncike ekhwalithini yama-antibodies kanye ne-reagent yokuthola.
Umuntu
Ukukhethwa kwe-antibody yokuhlolwa kwe-lateral flow assay kuthathwa njengokubalulekile futhi kufanele kubhekwe izinto ezimbalwa ngenkathi kukhethwa i-antibody ye-lateral flow assay. Isici sokuqala ukuthi kube nokutholakala okungcono kwe-antibody. Okwesibili, imininingwane ye-antigen kufanele ihlolwe futhi iqinisekiswe. Futhi, ukukhetha phakathi kwama-monoclonals noma ama-polyclonals lapho kukhethwa ama-antibody. Ama-monoclonals anconyelwa iqiniso lokuthi kungaba khona ukukhiqizwa kwenani elikhulu lama-antibody futhi ngaleyo ndlela kusize ekuboneni i-epitope eyodwa ye-antigenic kanti ama-Polycilonals angaba yisihloko sokwehlukahluka futhi ukunikezwa kwawo akuqinisekisiwe nakho.
Ulwelwesi lweNitcocellulose
Ulwelwesi lwe-Substrate noma ulwelwesi lweNitcocellulose kungenye yezinto ezibaluleke kakhulu futhi ezibaluleke kakhulu ekutholeni i-Lateral Flow Assay kanye nokubhekela ukuzwela kwayo. Ulayini wokuhlola nomugqa wokulawula udwetshiwe phezu kolwelwesi ukuhlinzeka ngokubopha okuhle ukubamba amasosha omzimba. Ukukhangiswa okungacacisiwe emigqeni yokuhlola nolayini bokulawula kungathinta ukuzwela kwemiphumela okungaveza imiphumela engamanga.
I-Adsorbent Pad
I-adsorbent Pad noma i-Wicking pad ibekwe ekugcineni komugqa we-strip noma we-lateral flow assay device. Ukuma kwenhloso kwephedi yokukhotha kungahle kube nesihlungi se-cellulose ukuthi i-helios ekwandiseni ubungako besampula emgqeni wokuhlola. Umsebenzi oyinhloko wephedi ye-adsorbent ukulawula ukuhamba kwesampula ketshezi phezu kolwelwesi lwe-nitrocellulose. Hhayi lokho kuphela, kodwa futhi kumisa ukugeleza okubuyela emuva kwesampula ngaleyo ndlela kugcinwe inani elanele lesifanekiso noma isampula.
Inqubo Izinketho ku I-lateral Flow Assay Mechanism
Noma isenzo sesinyathelo ngesinyathelo noma indlela ichazwe ngokuningiliziwe, izinketho zenqubo ku-lateral flow assay zibalulekile kuyo. Kunezinketho ezimbili zenqubo; eyodwa ukucubungula i-batch kanti enye isemhlanganweni we-line / reel-to-reel. Ukuqhutshwa kweqoqwana kusebenza ngempumelelo futhi kudinga abasebenzi abangaholela ekushintsheni kwemiphumela, ngaleyo ndlela kuveze imiphumela engathembekile. Ngenkathi i-reel-to-reel Assembly iyinketho yokucubungula ngomshini futhi inokwethenjelwa kakhulu ngobuningi obukhulu noma obuningi. Phakathi kwazo zombili izinhlobo zezinketho zokucubungula, isici sethempelesha sibaluleke kakhulu futhi kufanele sicatshangelwe ukuthi sinikeze imiphumela eyiqiniso futhi efiselekayo.
Isiphetho
I- Lateral Flow Assay Mechanism inezingxenye ezimbalwa ezihlanganisa ama-pad, ama-membrane, kanye nezinhlayiya ezithile ezisiza ukuthola isampula futhi zikhiqize imiphumela. Ngisho noma kunezinhlobo ezahlukene zamadivayisi e-Lateral Flow Assay futhi ezinye zazo zifakwe ngobuchwepheshe kanti ezinye azifakiwe kodwa indlela yokusebenza ihlala ifana. Zonke izici ezisetshenziswayo ze-Lateral Flow Assay kumele zibhekwe njengezibalulekile futhi akufanele zishaywe indiva.
Okubhekwayo
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test