I-lateral Flow Assay Mechanism of Action Isinyathelo ngesinyathelo

Ukugeleza Kwangemuva yokuncwaninga izinsimbi kanye namakhemikhali, ithuluzi noma ukuhlolwa kwemichilo okusetshenziselwa ukucacisa ubukhona noma ukungabikho komhlaziyi kusampula, kunendlela elandelwayo kuyo yonke inqubo yokuhlola. Umshini uqinisekisa ukusebenza kovivinyo, ukwenza inqubo, nokuthola imiphumela yokuhlolwa. Ukwengeza iqiniso, kufanele kwaziwe ukuthi ngisho nobuchwepheshe bebukuyo yonke indawo emkhakheni wezokwelapha futhi Ukuhlolisiswa Kokuhamba Kwamuva awehlukaniswa nalokho. Ngakho-ke, ubuchwepheshe bufakiwe kufayela le- Ukuhlanjululwa Kwesikhathi Esizayo kepha ukusebenza kwabo noma indlela abahlala ngayo iyafana. Futhi lesi ngesinye sezizathu okuyisona sixhobo sokuhlola esiphambili samazwe athuthukile nasathuthukayo.

Ukuqonda indlela yokusebenza kwe- Ukuhlanjululwa Kwesikhathi Esizayo, nangu ukubuka kafushane kwendlela esebenza ngayo. Okokuqala, kunephedi eyisampula lapho kulayishwa khona isifanekiso, futhi lokho kuqhubekela kuphedi yokuhlanganisa. Kuphedi yokuhlanganisa, izehlakalo ezibophayo zenzeka ngenxa yobukhona bezinhlayiya zokutholwa ezingaba yigolide noma i-latex. Isinyathelo esilandelayo esenzekayo ukuthi isampula ne-ejenti yokuphendula ibopha futhi iqhubekele kulwelwesi lokuphendula. Ulwelwesi lokuphendula lusiza ekukhiqizeni imiphumela. Ulayini wokuhlola uyama noma uqhubeke nokugeleza kuze kube yiphedi ye-adsorbent. Ukwazi ukuthi i-Lateral Flow Assay isebenze yini ama-reaction agents abopha kulayini wokulawula futhi akhiqize imiphumela. Indlela enemininingwane yeLateral Flow Assay ichazwa ngokulandelayo.

Isampuli Pad

Isampula Pad ukuqala kokuhlolwa kwe-assay flow lateral. Ukwakheka kwephedi yokufaka isampula yi-fiber fiber, i-mesh ephothiwe, noma i-cellulose. I-fiber fiber, i-mesh ephothiwe, ne-cellulose inezakhiwo ezihlukile komunye nomunye. Futhi, ukusebenza nezindleko zazo futhi kuya ngohlobo. Ngokuyisisekelo, iphedi lesampula lesampula liqala ukuhlolwa kwe-Lateral Flow Assay futhi umsebenzi oyinhloko walokhu ukuthola ubuningi besifanekiso noma isampula bese usabalalisa. Kubalulekile ukuthi isampula pad livumele bushelelezi besampula ukuze ikwazi ukwehlukanisa isampula, ukulungiswa kwe-pH, nokubopha ngokulinganayo. Ukuze isivivinyo siqaliswe, isampula kufanele lifakwe kuphedi yohlelo lokusebenza lwesampula.

Isihlanganisi Pad

Iphedi ye-conjugate iyisinyathelo sesibili sendlela yokulinganisa i-Lateral Flow Assay. Iphedi Yokukhulula I-Conjugate yakhiwe nge-fiber yengilazi engalukiwe futhi kukhona i-ejenti yokuthola eseyomile kakade. Ukuyixhumanisa nephedi yokufaka isampula, lapho isampula ifika endaweni yokugcwala futhi igeleza iye kuphedi yokukhulula i-conjugate futhi lapha i-ejenti yokuthola ikhishwa ukuze ihlaziye isampula. Umsebenzi oyinhloko wephedi ye-conjugate ukuqinisekisa ukuthi isampula kanye ne-ejenti yokusabela kuthuthelwa ku-membrane ngenkathi kugcinwa ne-conjugate. Kuphedi ye-conjugate, kumele kube nokubopha okuphansi okungaqondile ukuqinisekisa ukuthi i-reagent yokuthola ayinamathele kuphedi.

Ukutholwa kwe-Reagent

Kuhlala kutholakala i-reagent kufayela le- ukuhlolwa kokuhlolwa kwe-lateral flow. I-reagent yokuthola inama-antibodies ahlanganiswe kabusha ne-fluorescent noma izinhlayiya ezinemibala. Ukusebenza kwe-assay flow lateral kuncike kwikhwalithi yama-antibodies kanye ne-reagent reagent.

Umuntu

Ukukhethwa kwe-antibody yokuhlolwa kwe-lateral flow assay kuthathwa njengokubalulekile futhi kufanele kubhekwe izinto ezimbalwa ngenkathi kukhethwa i-antibody ye-lateral flow assay. Isici sokuqala ukuthi kube nokutholakala okungcono kwe-antibody. Okwesibili, imininingwane ye-antigen kufanele ihlolwe futhi iqinisekiswe. Futhi, ukukhetha phakathi kwama-monoclonals noma ama-polyclonals lapho kukhethwa ama-antibody. Ama-monoclonals anconyelwa iqiniso lokuthi kungaba khona ukukhiqizwa kwenani elikhulu lama-antibody futhi ngaleyo ndlela kusize ekuboneni i-epitope eyodwa ye-antigenic kanti ama-Polycilonals angaba yisihloko sokwehlukahluka futhi ukunikezwa kwawo akuqinisekisiwe nakho.

Ulwelwesi lweNitcocellulose

Ulwelwesi lwe-Substrate noma ulwelwesi lweNitcocellulose kungenye yezinto ezibaluleke kakhulu futhi ezibaluleke kakhulu ekutholeni i-Lateral Flow Assay kanye nokubhekela ukuzwela kwayo. Ulayini wokuhlola nomugqa wokulawula udwetshiwe phezu kolwelwesi ukuhlinzeka ngokubopha okuhle ukubamba amasosha omzimba. Ukukhangiswa okungacacisiwe emigqeni yokuhlola nolayini bokulawula kungathinta ukuzwela kwemiphumela okungaveza imiphumela engamanga.

I-Adsorbent Pad

I-adsorbent Pad noma i-Wicking pad ibekwe ekugcineni komugqa we-strip noma we-lateral flow assay device. Ukuma kwenhloso kwephedi yokukhotha kungahle kube nesihlungi se-cellulose ukuthi i-helios ekwandiseni ubungako besampula emgqeni wokuhlola. Umsebenzi oyinhloko wephedi ye-adsorbent ukulawula ukuhamba kwesampula ketshezi phezu kolwelwesi lwe-nitrocellulose. Hhayi lokho kuphela, kodwa futhi kumisa ukugeleza okubuyela emuva kwesampula ngaleyo ndlela kugcinwe inani elanele lesifanekiso noma isampula.

Inqubo Izinketho ku I-lateral Flow Assay Mechanism

Noma isenzo sesinyathelo ngesinyathelo noma indlela ichazwe ngokuningiliziwe, izinketho zenqubo ku-lateral flow assay zibalulekile kuyo. Kunezinketho ezimbili zenqubo; eyodwa ukucubungula i-batch kanti enye isemhlanganweni we-line / reel-to-reel. Ukuqhutshwa kweqoqwana kusebenza ngempumelelo futhi kudinga abasebenzi abangaholela ekushintsheni kwemiphumela, ngaleyo ndlela kuveze imiphumela engathembekile. Ngenkathi i-reel-to-reel Assembly iyinketho yokucubungula ngomshini futhi inokwethenjelwa kakhulu ngobuningi obukhulu noma obuningi. Phakathi kwazo zombili izinhlobo zezinketho zokucubungula, isici sethempelesha sibaluleke kakhulu futhi kufanele sicatshangelwe ukuthi sinikeze imiphumela eyiqiniso futhi efiselekayo.

Isiphetho

The I-lateral Flow Assay Mechanism iqukethe izingxenye ezimbalwa ezibandakanya amaphedi, ulwelwesi, nezinhlayiya ezithile ezisiza ukuthola isampula futhi zikhiqize imiphumela. Noma kunamadivayisi ahlukile weLateral Flow Assay futhi amanye awo afakiwe kwezobuchwepheshe ngenkathi amanye engekho kodwa indlela yokusebenza iyafana. Zonke izici ezisetshenziswayo ze-Lateral Flow Assay kumele zithathwe njengezibalulekile futhi akufanele neze zinganakwa.

Okubhekwayo
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://www.sciencedirect.com/topics/medicine-and-dentistry/lateral-flow-test