Ukuhlolwa Kokukhulelwa Kwesikhathi Esizayo nokuthi Kusebenza kanjani?
I-Lateral Flow Assay iyithuluzi elisetshenziselwa izinhlobo ezahlukene zokuhlola ngokuthola i-target analyte, uma ikhona noma ingekho kusampula. Amadivayisi e-Lateral Flow Assay asetshenziswa emhlabeni wonke ngezinzuzo eziningana futhi enye yezinzuzo ukuthi angasetshenziswa ekuhlolweni kwasekhaya. Ngokuphathelene nokuhlolwa kwasekhaya, umuntu angathola izifo, izifo, futhi enze nokuhlolwa kokukhulelwa ekhaya . Ukuhlolwa kwe-Lateral Flow Assay kusetshenziselwa ukuhlonza ama-biomarker kumchamo, ematheni, egazini, eplasma, nakwamanye amasampula ngezinjongo zokuxilonga. Ama-LFA avamile aveza imiphumela yokulinganisa noma yokulinganisa, edinga ukusetshenziswa kwezinsimbi ezikhethekile ukuthola imiphumela yokulinganisa. I-LFA iyindlela yokuhlola esebenzisa iphepha ukuthola nokulinganisa ama-analyte ezingxubeni eziyinkimbinkimbi. Isampula ibekwa kudivayisi yokuhlola, futhi imiphumela ivame ukutholakala phakathi nemizuzu emihlanu kuya kwengamashumi amathathu.
Izingxenye zamadivayisi wokugeleza kwe-lateral ziyisampula pad, i-conjugate pad, ulwelwesi lwe-nitrocellulose, ne-adsorbent pad. Lapho isampula noma i-matrix ifakwa kuphedi lesampula igelezela ngasephedi elihlanganisiwe lapho ama-antigen webhayoloji kanye nama-antibody aqala ukubopha nezinhlayiya ezivela kusampula pad lihlangana nama-nanoparticles kuphedi elihlanganisiwe. Okulandelayo, baya ngaselangeni. Lapha ama-nanoparticles aba yi-analyte eboshiwe futhi kudivayisi ye-Lateral Flow Assay imigqa emibili ilayini wokuhlola nolayini wokulawula. Lawa ma-nanoparticles azoqala ukudlulela ngaphesheya komugqa wokuhlola noma umugqa wokulawula.
Ulayini wokuhlola uqukethe ama-nanoparticles angenamandla angahlangana nezinhlayiya ezisepalini elihlanganisiwe ukukhombisa ukuba khona komhlaziyi ohlosiwe. Kodwa-ke, kusuka kulayini wokuhlola, uketshezi ludlulela kulayini wokulawula. Ulayini Wokulawula uqukethe imigqa yokusondelana esebenza ukuhlanganisa izinhlayiya ze-conjugate ne-analyte noma ngaphandle komhlaziyi ukukhombisa ukuthi idivayisi ye-lateral flow assay isebenza kahle futhi kahle. Manje, ngemuva kokuthi uketshezi seludlulisiwe kusuka kuyo yomibili imigqa okungukuthi umugqa wokuhlola nentambo yokulawula kukhona iphedi ye-adsorbent. I-Adsorbent pad isebenza ukukhangisa konke uketshezi olweqile. Njengoba inani elincane nelilawulwayo le-matrix liyadingeka, ngakho-ke, liqinisekisa ukuvumela ukuhamba kuketshezi okudingeka kuphela.
Ngesikhathi kwenziwa izivivinyo zokukhulelwa kumadivayisi okugeleza okusemaceleni angamafomethi amabili okungukuthi ifomethi yokuncintisana kanye nefomethi yesandwich. Ukusetshenziswa kwefomethi yokuncintisana kuhlonza ama-analyte uma kwenzeka ukuthi ama-antibodies noma i-analyte ayanele ukubopha okuningi. Kufomethi yokuncintisana, kunezinhlayiya ze-analyte emgqeni wokuhlola kanye ne-protein-analyte complex ene-conjugate pad etshela ukuthi kukhona noma akukho i-analyte. Kulolu hlobo lwefomethi, uma i-target analyte ingekho, ama-conjugates azobopha emgqeni wokuhlola. Uma sibheka ifomethi yesandwich yokuhlolwa kokukhulelwa kwamadivayisi okugeleza okusemaceleni, le fomethi isetshenziselwa ukuthola ama-target analyte, uma ekhona noma engekho. Ngaphezu kwalokho, la ma-analyte amakhulu kumele abe nama-epitopes. Futhi ama-antibodies ezindaweni ezimbili zokubhalisa zale fomethi abopha ngendlela i-analyte eyodwa ezobopha ngayo nezinhlayiya ezihlanganisiwe kanye nenye i-analyte enolunye ukuhlolwa kokubopha okuzosetshenziselwa umugqa wokuhlola we-lateral flow Assay. Kodwa-ke, uma i-target analyte itholakala izobopha ne-antibody-nanoparticle conjugate futhi iveze imiphumela efiselekayo. Futhi, iphuzu elibalulekile ukuthi kufomethi yesandwich yedivayisi ye-latera Flow essay yokuhlolwa kokukhulelwa, kuvela umugqa onemibala okusho ukuthi umphumela ulungile, Kodwa-ke, kufomethi yokuncintisana kuzoba nokungabikho/ukunyamalala kwemigqa enemibala kudivayisi okusho ukuthi imiphumela ilungile. kudivayisi yokuhlola ukugeleza kwe-lateral isebenza ngale ndlela yokwenza ukuhlolwa kokukhulelwa kuyo.
Ukuze kuhlaziywe isivivinyo sokugeleza kwe-lateral, kubalulekile ukuqaphela ukuthi izivivinyo zizoba sezimpawu ezingafundwa ngamehlo enyama futhi ziqondakala kalula. Ukuqinisekisa ukuzwela kwedivayisi yokuhlola ukugeleza kwe-lateral kubalulekile ukuba nesignali enamandla kakhulu ngangokunokwenzeka. Uma kunezinhlayiya ezinkulu ukubopha kuzoba namandla kodwa uma kuzoba kukhulu kakhulu kuzoba nomkhawulo ekugelezeni kwesampula. Imiphumela yedivayisi yokuhlola ukugeleza kwe-lateral izoba ngefomethi elandelayo okungukuthi, esezingeni eliphezulu, esezingeni eliphansi, kanye nelinobungako. Kodwa-ke, izivivinyo zokukhulelwa kudivayisi yokuhlola ukugeleza kwe-lateral, zizobonisa imiphumela esezingeni eliphezulu okungukuthi Yebo noma Cha. Ku-Lateral Flow Pregnancy Test , ukuhlaziywa kanye nemiphumela kusekelwe ekuhlaziyweni kwe -HCG . I-HCG imele i-Human chorionic gonadotropin eyakhiwe ngamaseli e-trophoblast. Lapho i-matrix ifakwa kuphedi yesampula yedivayisi, i-Human chorionic gonadotropin iboshelwe kuma-nanoparticles egolide ahlanganiswe ne-test antibody kanye ne-conjugated antibody. Ngenxa yokuthi lokhu kuwukuhlanganiswa okuvamile kwe-hCG emchameni wakho, amazinga ayo ayahlukahluka kuye ngokuthi uthatha nini isivivinyo. Umkhawulo wokuthola isivivinyo uvame ukuba phakathi kuka-20 no-100 mIU/mL. Uma sikhuluma ngokuxilonga okulinganiselwe, ukuqina komugqa wokuhlola kunqunywa yizinga elibekiwe okungukuthi elibizwa ngokuthi izinga lokulinganisa futhi kulokhu, ama-analyte aguqulwa abe yinani lokuhlushwa. Ukuze kutholakale imiphumela enembile, kuphakanyiswa ukusebenzisa i-strip reader ukufunda imiphumela kudivayisi ye-Lateral Flow Assay.
Isiphetho
Amadivayisi okuhlola i-Lateral Flow Assay ashibhile futhi kulula ukuwasebenzisa asetshenziswa amazwe athuthukile nasathuthuka. Ngaphezu kwalokho, amadivayisi okuhlola i-lateral flow angenye yezindlela zokuhlola ezithembeke kakhulu zokuhlola ukukhulelwa njengoba eveza imiphumela yangempela futhi cishe wonke umuntu uyazi ukuthi angawasebenzisa kanjani ngesikhathi esivumelana naye. Idivayisi yokuhlola i-Lateral Flow Assay iyona ehamba phambili ekwenzeni noma yiluphi uhlobo lokuhlola ekhaya.
Okubhekwayo
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4