Ukuhlolwa Kokukhulelwa Kwesikhathi Esizayo nokuthi Kusebenza kanjani?
Ukugeleza Kwangemuva I-Assay yidivayisi esetshenziselwa izinhlobo ezahlukahlukene zokuhlolwa ngokutholwa komhlaziyi wethagethi, uma ngabe ikhona noma cha kusampula. Amadivayisi we-Lateral Flow Assay zisetshenziselwa umhlaba wonke ngezinzuzo eziningana kanti enye yezinzuzo ukuthi zingasetshenziselwa ukuhlolwa kwasekhaya. Ngokuya ngokuhlolwa kwasekhaya, umuntu angathola izifo, ukutheleleka, futhi enze i- ukuhlolwa kokukhulelwa ekhaya. Ukuhlanjululwa Kwesikhathi Esizayo izivivinyo zisetshenziselwa ukukhomba ama-biomarker kumchamo, amathe, igazi, i-plasma, namanye amasampula ngezinjongo zokuxilonga. Ama-LFA avamile anikeza imiphumela elinganiselwe noma yekhwalithi, edinga ukusetshenziswa kwezinsimbi ezikhethekile ukuze kutholwe imiphumela yobuningi. I-LFA iyindlela yokuhlola esebenzisa iphepha ukuze kutholwe futhi kulinganise izihlaziyi ezixubeni eziyinkimbinkimbi. Isampula lifakwa kudivayisi yokuhlola, futhi imiphumela ivamise ukutholakala phakathi kwemizuzu emihlanu kuya kwengamashumi amathathu.
Izingxenye zamadivayisi wokugeleza kwe-lateral ziyisampula pad, i-conjugate pad, ulwelwesi lwe-nitrocellulose, ne-adsorbent pad. Lapho isampula noma i-matrix ifakwa kuphedi lesampula igelezela ngasephedi elihlanganisiwe lapho ama-antigen webhayoloji kanye nama-antibody aqala ukubopha nezinhlayiya ezivela kusampula pad lihlangana nama-nanoparticles kuphedi elihlanganisiwe. Okulandelayo, baya ngaselangeni. Lapha ama-nanoparticles aba yi-analyte eboshiwe futhi kudivayisi ye-Lateral Flow Assay imigqa emibili ilayini wokuhlola nolayini wokulawula. Lawa ma-nanoparticles azoqala ukudlulela ngaphesheya komugqa wokuhlola noma umugqa wokulawula.
Ulayini wokuhlola uqukethe ama-nanoparticles angenamandla angahlangana nezinhlayiya ezisepalini elihlanganisiwe ukukhombisa ukuba khona komhlaziyi ohlosiwe. Kodwa-ke, kusuka kulayini wokuhlola, uketshezi ludlulela kulayini wokulawula. Ulayini Wokulawula uqukethe imigqa yokusondelana esebenza ukuhlanganisa izinhlayiya ze-conjugate ne-analyte noma ngaphandle komhlaziyi ukukhombisa ukuthi idivayisi ye-lateral flow assay isebenza kahle futhi kahle. Manje, ngemuva kokuthi uketshezi seludlulisiwe kusuka kuyo yomibili imigqa okungukuthi umugqa wokuhlola nentambo yokulawula kukhona iphedi ye-adsorbent. I-Adsorbent pad isebenza ukukhangisa konke uketshezi olweqile. Njengoba inani elincane nelilawulwayo le-matrix liyadingeka, ngakho-ke, liqinisekisa ukuvumela ukuhamba kuketshezi okudingeka kuphela.
Ngenkathi wenza izivivinyo zokukhulelwa ku- amadivayisi we-lateral flow Okuyifomethi ezimbili okungukuthi ifomethi yokuncintisana kanye nefomethi yesandwich. Ukusetshenziswa kwefomethi yokuncintisana kuhlonza ama-analyte uma kwenzeka ukuthi ama-antibodies noma i-analyte ayanele ukubopha okuningi. Kufomethi yokuncintisana, kunezinhlayiya ze-analyte emgqeni wokuhlola kanye ne-protein-analyte complex ene-conjugate pad etshela ukuthi kukhona noma akukho i-analyte. Kulolu hlobo lwefomethi, uma i-target analyte ingekho, ama-conjugates azobopha emgqeni wokuhlola. Uma sibheka ifomethi yesandwich yokuhlolwa kokukhulelwa kwamadivayisi okugeleza okuqondile, le fomethi isetshenziselwa ukuthola ama-target analyte, uma ekhona noma engekho. Ngaphezu kwalokho, la ma-analyte amakhulu kumele abe nama-epitopes. Futhi ama-antibodies ezindaweni ezimbili zokubhalisa zale fomethi abopha ngendlela i-analyte eyodwa ezobopha ngayo nezinhlayiya ezihlanganisiwe kanye nenye i-analyte enolunye ukuhlolwa kokubopha okuzosetshenziselwa umugqa wokuhlola we-lateral flow Assay. Kodwa-ke, uma i-target analyte itholakala izobopha ne-antibody-nanoparticle conjugate futhi iveze imiphumela efiselekayo. Futhi, iphuzu elibalulekile ukuthi kufomethi yesandwich yedivayisi ye-latera Flow essay yokuhlolwa kokukhulelwa, kuvela umugqa onemibala okusho ukuthi umphumela ulungile, Kodwa-ke, kufomethi yokuncintisana kuzoba nokungabikho/ukunyamalala kwemigqa enemibala kudivayisi okusho ukuthi imiphumela ilungile. kudivayisi yokuhlola ukugeleza kwe-lateral isebenza ngale ndlela yokwenza ukuhlolwa kokukhulelwa kuyo.
Ukuhlaziywa kokuhlolwa kwe-lateral flow assay test, kubalulekile ukuthi wazi ukuthi ukuhlolwa kungaba kuzimpawu ezingafundwa ngamehlo futhi kuqondakale kalula. Ukuqinisekisa ukuzwela kwensimbi ye-lateral flow assay kubalulekile ukuthi ube nesiginali enamandla kunayo yonke engenzeka ngezinga. Uma kunezinhlayiya ezinkulu ukubopha kuzoqina kepha uma kuzoba kukhulu kakhulu kuzoba nenkinga ekuhambeni kwesampula. Imiphumela yedivayisi ye-lateral flow assay izoba ngamafomethi alandelayo okungukuthi ikhwalithi, i-semi-qualitative, nobungako. Kodwa-ke, ukuhlolwa kokukhulelwa kudivayisi ye-lateral flow assay, kuzobonisa imiphumela yekhwalithi okungukuthi Yebo noma Cha Ukuhlolwa Kokukhulelwa Kwesikhathi Esizayo, ukuhlaziywa kanye nemiphumela kuncike ku- I-HCG ukuhlaziywa. I-HCG imele i-Human chorionic gonadotropin eyakhiwe ngamaseli we-trophoblast. Lapho i-matrix ifakwa kusampuli yedivayisi, i-Human chorionic gonadotropin iboshelwe kuma-nanoparticles egolide ahlanganiswe ne-antibody yokuhlola kanye ne-antibody ehlanganisiwe. Ngoba lokhu kungukuhlanganiswa okujwayelekile kwe-hCG emchameni wakho, izingqinamba zayo ziyahlukahluka kuye ngokuthi uthatha nini isivivinyo. Umkhawulo wokuthola isivivinyo uvame ukuba phakathi kwama-20 no-100 mIU / mL. Ukukhuluma ngokuxilongwa kobuningi, ukuqina komugqa wokuhlola kunqunywa yizinga elijwayelekile elibekiwe elibizwa ngokuthi isilinganiso sokulinganisa futhi kulokhu, izibalo ziguqulwa zibe inani lokuhlushwa. Ngokunquma imiphumela enembile, kuphakanyiswa ukuthi kusetshenziswe i-strip reader ukufunda imiphumela kudivayisi ye-Lateral Flow Assay.
Isiphetho
Amadivayisi we-Lateral Flow Assay angabizi futhi asebenziseka kalula amadivayisi asetshenziswa amazwe athuthukile nasathuthukayo. Ngaphezu kwalokho, amadivayisi we-lateral flow angenye yezindlela zokuhlola ezinokwethenjelwa kakhulu ze- ukuhlolwa kokukhulelwa njengoba kuveza imiphumela eyiqiniso futhi cishe wonke umuntu uyazi ukuthi angayisebenzisa kanjani ngesikhathi sakhe esivumayo. Idivayisi ye-Lateral Flow Assay iyona ehamba phambili kakhulu yokwenza noma yiluphi uhlobo lokuhlola ekhaya.
Okubhekwayo
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4