Ukugeleza Kwangemuva I-Assay yidivayisi esetshenziselwa izinhlobo ezahlukahlukene zokuhlolwa ngokutholwa komhlaziyi wethagethi, uma ngabe ikhona noma cha kusampula. Amadivayisi we-Lateral Flow Assay zisetshenziselwa umhlaba wonke ngezinzuzo eziningana kanti enye yezinzuzo ukuthi zingasetshenziselwa ukuhlolwa kwasekhaya. Ngokuya ngokuhlolwa kwasekhaya, umuntu angathola izifo, ukutheleleka, futhi enze i- ukuhlolwa kokukhulelwa ekhaya. Ukuhlanjululwa Kwesikhathi Esizayo izivivinyo zisetshenziselwa ukukhomba ama-biomarker kumchamo, amathe, igazi, i-plasma, namanye amasampula ngezinjongo zokuxilonga. Ama-LFA avamile anikeza imiphumela elinganiselwe noma yekhwalithi, edinga ukusetshenziswa kwezinsimbi ezikhethekile ukuze kutholwe imiphumela yobuningi. I-LFA iyindlela yokuhlola esebenzisa iphepha ukuze kutholwe futhi kulinganise izihlaziyi ezixubeni eziyinkimbinkimbi. Isampula lifakwa kudivayisi yokuhlola, futhi imiphumela ivamise ukutholakala phakathi kwemizuzu emihlanu kuya kwengamashumi amathathu.
Izingxenye zamadivayisi wokugeleza kwe-lateral ziyisampula pad, i-conjugate pad, ulwelwesi lwe-nitrocellulose, ne-adsorbent pad. Lapho isampula noma i-matrix ifakwa kuphedi lesampula igelezela ngasephedi elihlanganisiwe lapho ama-antigen webhayoloji kanye nama-antibody aqala ukubopha nezinhlayiya ezivela kusampula pad lihlangana nama-nanoparticles kuphedi elihlanganisiwe. Okulandelayo, baya ngaselangeni. Lapha ama-nanoparticles aba yi-analyte eboshiwe futhi kudivayisi ye-Lateral Flow Assay imigqa emibili ilayini wokuhlola nolayini wokulawula. Lawa ma-nanoparticles azoqala ukudlulela ngaphesheya komugqa wokuhlola noma umugqa wokulawula.
Ulayini wokuhlola uqukethe ama-nanoparticles angenamandla angahlangana nezinhlayiya ezisepalini elihlanganisiwe ukukhombisa ukuba khona komhlaziyi ohlosiwe. Kodwa-ke, kusuka kulayini wokuhlola, uketshezi ludlulela kulayini wokulawula. Ulayini Wokulawula uqukethe imigqa yokusondelana esebenza ukuhlanganisa izinhlayiya ze-conjugate ne-analyte noma ngaphandle komhlaziyi ukukhombisa ukuthi idivayisi ye-lateral flow assay isebenza kahle futhi kahle. Manje, ngemuva kokuthi uketshezi seludlulisiwe kusuka kuyo yomibili imigqa okungukuthi umugqa wokuhlola nentambo yokulawula kukhona iphedi ye-adsorbent. I-Adsorbent pad isebenza ukukhangisa konke uketshezi olweqile. Njengoba inani elincane nelilawulwayo le-matrix liyadingeka, ngakho-ke, liqinisekisa ukuvumela ukuhamba kuketshezi okudingeka kuphela.
Ngenkathi wenza izivivinyo zokukhulelwa ku- amadivayisi we-lateral flow lawo amafomethi amabili okungukuthi ifomethi yokuncintisana nefomethi yesangweji. Ukusetshenziswa kwefomethi yokuncintisana kungukutholwa kwama-analyte uma kwenzeka amasosha omzimba noma i-analyte enganele ukubopha okuningi. Ngefomethi yokuncintisana, kukhona ama-molecule we-analyte kulayini wokuhlola kanye ne-protein-analyte eyinkimbinkimbi nephedi ye-conjugate etshela abt ubukhona noma ukungabikho kwe-analyte. Kulolu hlobo lwefomethi, uma umhlaziyi wethagethi engekho, ama-conjugates azobopheka kulayini wokuhlola. Uma ufika kufomethi yesandwich yokuhlolwa kokukhulelwa kwamadivayisi we-lateral flow, le fomethi isetshenziselwa ukutholwa kwama-analyties ahlosiwe, uma akhona noma engekho, lawa ma-analytic amakhulu kumele abe nama-epitopes. Futhi amasosha omzimba asezindaweni ezimbili zokubhoda zale fomethi abopha ngendlela yokuthi umhlaziyi oyedwa abophe izinhlayiya ezihlanganisiwe nomunye umhlaziyi nolunye uhlolo olubophayo oluzosetshenziselwa umugqa wokuhlola we-lateral flow Assay. Kodwa-ke, uma kutholakala umhlaziyi ohlosiwe uzobopha ne-anti-nanoparticle conjugate bese ekhipha imiphumela oyifunayo. Futhi, iphuzu elibalulekile ukuthi kwifomethi yesamishi yedivayisi ye-latera Flow eseyi yokuhlolwa kokukhulelwa, umugqa onemibala uyavela okusho ukuthi umphumela ulungile, kepha, kufomethi yokuncintisana kuzoba khona ukungabikho / ukunyamalala kwemigqa enemibala kudivayisi okusho ukuthi imiphumela iba mihle. kudivayisi ye-lateral flow assay isebenza ngale ndlela ngokwenza ukuhlolwa kokukhulelwa kuyo.
Ukuhlaziywa kokuhlolwa kwe-lateral flow assay test, kubalulekile ukuthi wazi ukuthi ukuhlolwa kungaba kuzimpawu ezingafundwa ngamehlo futhi kuqondakale kalula. Ukuqinisekisa ukuzwela kwensimbi ye-lateral flow assay kubalulekile ukuthi ube nesiginali enamandla kunayo yonke engenzeka ngezinga. Uma kunezinhlayiya ezinkulu ukubopha kuzoqina kepha uma kuzoba kukhulu kakhulu kuzoba nenkinga ekuhambeni kwesampula. Imiphumela yedivayisi ye-lateral flow assay izoba ngamafomethi alandelayo okungukuthi ikhwalithi, i-semi-qualitative, nobungako. Kodwa-ke, ukuhlolwa kokukhulelwa kudivayisi ye-lateral flow assay, kuzobonisa imiphumela yekhwalithi okungukuthi Yebo noma Cha Ukuhlolwa Kokukhulelwa Kwesikhathi Esizayo, ukuhlaziywa kanye nemiphumela kuncike ku- I-HCG ukuhlaziywa. I-HCG imele i-Human chorionic gonadotropin eyakhiwe ngamaseli we-trophoblast. Lapho i-matrix ifakwa kusampuli yedivayisi, i-Human chorionic gonadotropin iboshelwe kuma-nanoparticles egolide ahlanganiswe ne-antibody yokuhlola kanye ne-antibody ehlanganisiwe. Ngoba lokhu kungukuhlanganiswa okujwayelekile kwe-hCG emchameni wakho, izingqinamba zayo ziyahlukahluka kuye ngokuthi uthatha nini isivivinyo. Umkhawulo wokuthola isivivinyo uvame ukuba phakathi kwama-20 no-100 mIU / mL. Ukukhuluma ngokuxilongwa kobuningi, ukuqina komugqa wokuhlola kunqunywa yizinga elijwayelekile elibekiwe elibizwa ngokuthi isilinganiso sokulinganisa futhi kulokhu, izibalo ziguqulwa zibe inani lokuhlushwa. Ngokunquma imiphumela enembile, kuphakanyiswa ukuthi kusetshenziswe i-strip reader ukufunda imiphumela kudivayisi ye-Lateral Flow Assay.
Isiphetho
Amadivayisi we-Lateral Flow Assay angabizi futhi asebenziseka kalula amadivayisi asetshenziswa amazwe athuthukile nasathuthukayo. Ngaphezu kwalokho, amadivayisi we-lateral flow angenye yezindlela zokuhlola ezinokwethenjelwa kakhulu ze- ukuhlolwa kokukhulelwa njengoba kuveza imiphumela eyiqiniso futhi cishe wonke umuntu uyazi ukuthi angayisebenzisa kanjani ngesikhathi sakhe esivumayo. Idivayisi ye-Lateral Flow Assay iyona ehamba phambili kakhulu yokwenza noma yiluphi uhlobo lokuhlola ekhaya.
Okubhekwayo
https://nanocomposix.com/pages/introduction-to-lateral-flow-rapid-test-diagnostics
https://studentsxstudents.com/pregnancy-tests-covid-19-testing-an-explanation-of-lateral-flow-assays-b45282ebdda4
