Umhlahlandlela Wokuxazulula inkinga we-Lateral Flow Assay

Ukuhlolwa kokugeleza kwe-lateral kuyithuluzi lokuhlola ukunakekelwa elihlonyiswe ngobuchwepheshe. Njengoba lihlonyiswe ngobuchwepheshe, kunethuba lokuthi kube nanoma iyiphi inkinga noma iphutha elingaba khona elingadida abasebenzisi balo bokugcina. Ukuze kuxazululwe inkinga, kanye nokuhlaziywa kwayo kubalulekile ukwazi ngenqubo yokuxazulula inkinga ye- Lateral Flow Assay . Ukuhlolwa kokugeleza kwe-lateral kungase kube nezinkinga ezithile ezifana nemiphumela emihle, imiphumela emibi, izinkinga ze-membrane, nezinye eziningi.

Umhlahlandlela wokuxazulula izinkinga zokuhlolwa kokugeleza kwe-lateral uba yisidingo uma kukhona ukuqinisekiswa kokwehluleka okubikiwe. Ekuhlulekeni okubikiwe, umuntu kumele achaze ukwehluleka ekuhlolweni kokuhlolwa kokugeleza kwe-lateral ukuze kube nolwazi olwanele. Ngaphezu kwalokho, kubalulekile ukwazi ukuthi ukwehluleka kwenzeka kangaki, inani lezikhathi okwenzeke ngazo, nokuthi isilinganiso salokhu kwehluleka sizophinde senzeke.

Kumhlahlandlela wokuxazulula izinkinga, kunezinyathelo eziningana ezinezinyathelo ezengeziwe okumele zilandelwe uma kwenzeka inkinga iphakama ekuhlolweni kokugeleza kwe-lateral kanye nedivayisi. Isinyathelo sokuqala ukuphenya izisekelo ze-LFA. Kulesi sinyathelo, umlando wezinto ezibonakalayo, kanye nokuguquguquka kwenqubo yokuhlolwa kwe-LFA kumele kuhlolwe. Futhi, uma kukhona ukubonwa okungavamile okuqashelwe ngenkathi kwenziwa ukuhlolwa, kuzoba wusizo ekutholeni inkinga. Manje isinyathelo sesibili ukuqala ukuhlolwa ngedivayisi esivele isiqediwe. Ngalokhu, kuhloswe ukuhlola idivayisi kahle ngaphambi kokuyithatha kunoma yiluphi uhlobo lokuhlolwa. Kufanele uhlole futhi uhlole imichilo. Ngesikhathi uhlola imichilo, kunezinto ezimbalwa okufanele zikhunjulwe njengokuhlola ukuhambisana kokuhlanganiswa kwezivivinyo, ukungcola ku-LFA, kanye nokuthi kukhona yini umonakalo ongokwenyama kudivayisi.

Isinyathelo esilandelayo sithatha indawo yezinto ezaziwa ngokusebenzela ukushintshanisa lezo ezingasebenzi ngaleso sikhathi. Ngale njongo, ungahle udinge ukubheka amasethi wamadivayisi wangaphambilini uma inkinga etholakele isedivayisini noma kunoma iyiphi ingxenye ethile yaleyo divayisi ye-LFA. Okufanayo kuya esimweni sanoma yiziphi izinto zamakhemikhali noma zebhayoloji okucatshangwa ukuthi ziyimbangela yenkinga lapho-ke kufanele zifakwe endaweni yenqwaba yamadivayisi ukunqoba le nkinga futhi.

Isinyathelo esilandelayo esiza kule nqubo ukuphenya ukushesha kwesilingo. Ukuphenya ijubane le-assay ungasebenzisa i-assay yesigamu. Ngosizo lwayo, kuba lula ukuxazulula izingqinamba ukuthi ngabe ziyizakhi zamakhemikhali noma izingxenye zebhayoloji. Ngokusebenzisa le ndlela, ukwahluka kusuka kumaphakethe we-conjugate, amasampula wesampula, izindlu, nezinqubo zomhlangano ezihlobene kungasuswa. Futhi iphuzu lokuhlanganisa ezinye izinto eziguquguqukayo zingahlolwa ngasikhathi sinye.

Ezinye izinto ezihilelekile kumhlahlandlela wokuxazulula izinkinga we- Lateral Flow Assay abathengisi, ukukhiqiza kanye ne-QC, kanye nabasebenzisi bomkhiqizo. Ngabathengisi, banesibopho sokukhiqiza ikhwalithi ngokuvumelana nokwengeza imininingwane ehambisana nokuhlolwa kokugeleza kwe-lateral. Ngaphezu kwalokho, inqubo yokukhiqiza kanye nokulawulwa kwekhwalithi kumele kuhlolwe njengoba lesi kuyisinyathelo esiyinhloko nesiyisisekelo ekukhiqizweni kwamadivayisi asezingeni elihle. Ukuqeqeshwa okuthuthukisiwe kumele kunikezwe ngezinqubo ezilungisiwe kanye nekhwalithi yomkhiqizo elungisiwe. Okokugcina, kubasebenzisi bokugcina, imiyalelo yokusebenza kumele ibhalwe ngokucacile nangokucacile ukuze baqonde ukusebenzisa idivayisi ngendlela enomthwalo wemfanelo.

Ngenkathi usebenzisa i-lateral flow assay, kukhona kungenzeka ukuthi ubone imiphumela emihle yamanga. Umphumela wokukhishwa kwamanga kwenzeka lapho umhlaziyi oyifunayo kusampula noma i-matrix engekho okwamanje imiphumela iphume kahle. Ukuxazulula le nkinga kubalulekile ukuqonda izimbangela ezahlukahlukene ngemuva kwayo. Izizathu zingaba ukubopha okungacacisiwe, ukubopha i-heterophilic, noma ukusebenza kabusha okuphambene nalokho. Ukuze kunqotshwe lezi zingqinamba, ukwenziwa kokuhlolwa kwe-lateral flow noma kwephedi yokuhlanganisa kufanele kwenziwe. Izimbangela ezibalulwe ngenhla zehluke ngokuphelele komunye nomunye ngokuya ngobumbano, ama-antigen, nama-antibodies. Futhi, ukuba khona kwama-antibacterial heterophilic kungaholela ekubophweni kwama-antibody nomhlaziyi ongahlosiwe, ngaleyo ndlela kube nemiphumela yamanga. Uma ekuhlolweni kwe-lateral flow assay yakho kunama-antibacterial heterophilic ungayixazulula inkinga ngokuthenga ama-reagents wokuvimbela i-heterophilic noma i-conjugate yegundane ingasetshenziswa ukuxazulula le nkinga ye-lateral flow assay.

Enye inkinga etholakala ku-lateral flow assay ukwehla kolawulo kanye nolayini wokuhlola lapho isampula noma i-matrix ifakwa kuphedi lesampula kudivayisi ye-LFA. Le nkinga ibonwe ezimweni eziningi futhi isizathu esivezwe kulokhu yizici ezahlukahlukene ku-matrix yomtholampilo noma isampula. Ukuxazulula le nkinga, ukusetshenziswa kwama-ejenti wokuvimba kuphedi yokuxuba noma yokuhlanganisa kungasiza ukunqoba le nkinga. Ama-ejenti ahlukile okuvimba angaba amaprotheni, usawoti, ama-molecule, noma ama-metabolites.

Isiphetho:

Ukuhlolwa Kokugeleza Kwe-Lateral yiwona madivayisi asetshenziswa kakhulu emhlabeni jikelele. Kodwa-ke, kungaba nezinkinga ngenxa yezizathu ezahlukene umuntu angabhekana nazo ngenkathi esebenzisa idivayisi. Umhlahlandlela wokuxazulula izinkinga wokuhlolwa kokugeleza kwe-lateral kusiza ukukhombisa ukuthi yini engenziwa ukuze kuhlaziywe inkinga futhi kuxazululwe kabanzi. Ekuphetheni kwazo zonke izinto ezibhalwe ngenhla, kumele kuthiwe ukuze kukhiqizwe ngempumelelo ukuhlolwa kokugeleza kwe-lateral kanye nemichilo yokuhlola ukugeleza kwe-lateral, kunenhlanganisela yezinkinga zebhayoloji, zamakhemikhali, zomzimba, kanye nezobunjiniyela. Kunconywa futhi kufanele kuthathwe isinyathelo sokucabangela zonke izici ezincane kanye nezici ngenkathi kukhiqizwa umkhiqizo ukuze kuncishiswe noma yiluphi uhlobo lokushintsha uma idivayisi kanye nemichilo kungaveza imiphumela engalungile ekugcineni. Futhi, uma kunenkinga nge-nitrocellulose membrane yedivayisi yokuhlola ukugeleza kwe-lateral, inkinga ingaba sekwakhiweni kwedivayisi noma kwezinye izinkinga zokuhlanganisa.

Okubhekwayo

https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf

https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes

https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting