Umhlahlandlela Wokuxazulula inkinga we-Lateral Flow Assay

Ukugeleza kwesikhathi Izilingo ayiphuzu lamadivayisi wokuhlola ukunakekelwa afakwe ubuchwepheshe. Njengoba zihlonyelwe ezobuchwepheshe, kunethuba lokuthi noma iyiphi inkinga noma iphutha okungenzeka liqhubeke nokudida abasebenzisi bayo bokugcina. Ngesixazululo senkinga, nokuhlaziywa kwayo kubalulekile ukwazi ngefayela le- inqubo yokuxazulula inkinga ye Ukuhlanjululwa Kwesikhathi Esizayo. I-Lateral Flow Assay ingahle ibe nezinkinga ezithile njengemiphumela enamanga, imiphumela engamanga, izinkinga ze-membrane, nezinye eziningi.

The umhlahlandlela wokuxazulula inkinga we-lateral flow assay iba yisidingo lapho kunesiqinisekiso sokwehluleka okubikiwe. Ekuhlulekeni okubikiwe, umuntu kufanele achaze ukwehluleka ekuhlolweni kwe-lateral flow assay test ukuze kube nemininingwane eyanele. Ngaphezu kwalokho, kubalulekile ukwazi imvamisa yokwehluleka, inani lezikhathi okwenzekile ngalo, nokuthi ikuphi isilinganiso salokhu kwehluleka okuzokwenzeka futhi.  

Kumhlahlandlela wokuxazulula inkinga, kunezinyathelo ezimbalwa ezinezinyathelo ezengeziwe okufanele zilandelwe uma kwenzeka inkinga ivela ukuhlolwa kokuhlolwa kwe-lateral flow nedivayisi. Isinyathelo sokuqala ukuphenya izisekelo ze-LFA. Kulesi sinyathelo, kufanele kuhlolwe umlando wezinto ezibonakalayo, kanye nokwehluka kwenqubo. Futhi, uma kukhona ukubonwa okungajwayelekile okubonwe ngenkathi kwenziwa isivivinyo, kuzosiza futhi ekunqumeni inkinga. Manje isinyathelo sesibili ukuqala ukuhlolwa ngedivayisi esevele isiqedile. Ngalokhu, kuhloselwe ukuhlola idivayisi kahle ngaphambi kokuyithathela noma yiluphi uhlobo lokuhlola. Kufanele uhlole futhi uhlole imichilo. Ngenkathi uhlola imichilo, kunokucatshangelwa okumbalwa okufanele kugcinwe engqondweni njengokuhlola ukungaguquguquki kokuhlangana kwezilingo, ukungcoliswa kwe-LFA, futhi uma kukhona ukulimala emzimbeni.

Isinyathelo esilandelayo sithatha indawo yezinto ezaziwa ngokusebenzela ukushintshanisa lezo ezingasebenzi ngaleso sikhathi. Ngale njongo, ungahle udinge ukubheka amasethi wamadivayisi wangaphambilini uma inkinga etholakele isedivayisini noma kunoma iyiphi ingxenye ethile yaleyo divayisi ye-LFA. Okufanayo kuya esimweni sanoma yiziphi izinto zamakhemikhali noma zebhayoloji okucatshangwa ukuthi ziyimbangela yenkinga lapho-ke kufanele zifakwe endaweni yenqwaba yamadivayisi ukunqoba le nkinga futhi.

Isinyathelo esilandelayo esiza kule nqubo ukuphenya ukushesha kwesilingo. Ukuphenya ijubane le-assay ungasebenzisa i-assay yesigamu. Ngosizo lwayo, kuba lula ukuxazulula izingqinamba ukuthi ngabe ziyizakhi zamakhemikhali noma izingxenye zebhayoloji. Ngokusebenzisa le ndlela, ukwahluka kusuka kumaphakethe we-conjugate, amasampula wesampula, izindlu, nezinqubo zomhlangano ezihlobene kungasuswa. Futhi iphuzu lokuhlanganisa ezinye izinto eziguquguqukayo zingahlolwa ngasikhathi sinye.

Ezinye izinto ezibandakanyekayo kumhlahlandlela wokuxazulula inkinga we Ukuhlanjululwa Kwesikhathi Esizayo ngabathengisi, abezokukhiqiza kanye ne-QC, nabasebenzisi bokugcina bomkhiqizo. Ngabathengisi, banesibopho sokukhiqiza ikhwalithi ngokungaguquguquki futhi bangeze imininingwane ebhekane nokuhlolwa kwe-lateral flow. Ngaphezu kwalokho, inqubo yokukhiqiza nokulawulwa kwekhwalithi kufanele kuhlolwe njengoba lesi kuyisinyathelo esikhulu nesisekelo ekukhiqizeni amadivayisi wekhwalithi enhle. Ukuqeqeshwa okuthuthukisiwe kufanele kunikezwe izinqubo ezithuthukisiwe kanye nekhwalithi elicwengekile yomkhiqizo. Okokugcina, kubasebenzisi bokugcina, imiyalo yokusebenza kufanele ibhalwe ngokusobala nangokucacile ukuze baqonde ukusebenzisa idivayisi ngendlela efanele.

Ngenkathi usebenzisa i-lateral flow assay, kukhona kungenzeka ukuthi ubone imiphumela emihle yamanga. Umphumela wokukhishwa kwamanga kwenzeka lapho umhlaziyi oyifunayo kusampula noma i-matrix engekho okwamanje imiphumela iphume kahle. Ukuxazulula le nkinga kubalulekile ukuqonda izimbangela ezahlukahlukene ngemuva kwayo. Izizathu zingaba ukubopha okungacacisiwe, ukubopha i-heterophilic, noma ukusebenza kabusha okuphambene nalokho. Ukuze kunqotshwe lezi zingqinamba, ukwenziwa kokuhlolwa kwe-lateral flow noma kwephedi yokuhlanganisa kufanele kwenziwe. Izimbangela ezibalulwe ngenhla zehluke ngokuphelele komunye nomunye ngokuya ngobumbano, ama-antigen, nama-antibodies. Futhi, ukuba khona kwama-antibacterial heterophilic kungaholela ekubophweni kwama-antibody nomhlaziyi ongahlosiwe, ngaleyo ndlela kube nemiphumela yamanga. Uma ekuhlolweni kwe-lateral flow assay yakho kunama-antibacterial heterophilic ungayixazulula inkinga ngokuthenga ama-reagents wokuvimbela i-heterophilic noma i-conjugate yegundane ingasetshenziswa ukuxazulula le nkinga ye-lateral flow assay.

Enye inkinga etholakala ku-lateral flow assay ukwehla kolawulo kanye nolayini wokuhlola lapho isampula noma i-matrix ifakwa kuphedi lesampula kudivayisi ye-LFA. Le nkinga ibonwe ezimweni eziningi futhi isizathu esivezwe kulokhu yizici ezahlukahlukene ku-matrix yomtholampilo noma isampula. Ukuxazulula le nkinga, ukusetshenziswa kwama-ejenti wokuvimba kuphedi yokuxuba noma yokuhlanganisa kungasiza ukunqoba le nkinga. Ama-ejenti ahlukile okuvimba angaba amaprotheni, usawoti, ama-molecule, noma ama-metabolites.

Isiphetho:

Ukuhlolisiswa Kokuhamba Kwamuva amadivayisi asetshenziswa kakhulu futhi asetshenziswa emhlabeni jikelele. Noma kunjalo, kungahle kube nezinkinga ngenxa yezizathu ezahlukahlukene umuntu angahlangabezana nazo ngenkathi esebenzisa le divayisi. The umhlahlandlela wokuxazulula inkinga we-lateral flow assay isiza ukukhombisa okungenziwa ukuhlaziya inkinga nokuyixazulula ngokuqhubekayo. Ekuphetheni konke okubhalwe ngenhla, kufanele sisho ukuthi ekwenziweni ngempumelelo kwezilingo zokuhamba kwe-lateral kanye nemichilo yokuhlolwa kokuhamba, kunenhlanganisela yezindaba zebhayoloji, zamakhemikhali, zomzimba nezobunjiniyela. Kunconywa futhi kufanele uthathe isinyathelo ukucubungula zonke izici nezici ezincane ngenkathi kukhiqizwa umkhiqizo ukunciphisa noma yiluphi uhlobo lokwehluka uma idivaysi nemichilo ingaveza imiphumela emibi ekugcineni. Futhi, uma kunenkinga nge-nitrocellulose membrane yedivayisi ye-lateral flow assay inkinga inkinga ingaba ekwakhiweni kwedivayisi noma ezinye izingqinamba zomhlangano.

Okubhekwayo

https://www.emdmillipore.com/INTERSHOP/static/WFS/Merck-Site/-/Merck/en_US/Freestyle/DIV-Divisional/Events/pdfs/lateral-flow-presentations/troubleshooting-lateral-flow-tests.pdf

https://www.cytivalifesciences.com/en/us/solutions/lab-filtration/knowledge-center/lateral-flow-assay-troubleshooting-and-how-to-switch-membranes

https://nanocomposix.com/pages/lateral-flow-tips-tricks-and-frequently-asked-questions-faq#troubleshooting