Yini Eyenza Ama-antibody Ahlale Ku-Nitrocellulose Membrane Lateral Flow Flow Assay?
I-Nitrocellulose Membrane ku- Lateral Flow Assay iza ngemuva kwesampula kanye ne-conjugate pad. I-Nitrocellulose membrane ibizwa nangokuthi i-substrate membrane futhi ingenye yezingxenye ezibaluleke kakhulu nezibucayi emiphumeleni yokuhlolwa okwenziwe ku- Lateral Flow Assay . Ku-Lateral Flow Assay, kungaba nezinto ezahlukene ezisetshenziswayo njenge-cellulose acetate, i-polyvinylidene fluoride (PVDF), i-charge modified nylon, kanye ne-polyethersulfone (PES), njll. Ku-Nitrocellulose membrane, kunosayizi we-pore ohlukile, i-porosity, ukujiya, kanye nokwelashwa ngamakhemikhali . I-Nitrocellulose membranes ayinawo ukusatshalaliswa okulinganayo kwe-pores ngakho-ke isikhathi sokugeleza kwe-capillary sithandwa kakhulu. Ngaphambi kokuqhubekela phambili ekucabangeni kubalulekile ukwazi ngokugeleza kwe-capillary. Ngokuyisisekelo, isikhathi sokugeleza kwe-capillary silinganiswa ngemizuzwana noma ngamasentimitha futhi yisikhathi esiphelele isampula esithathiwe ukuze sigcwaliswe ngokuphelele ku-test strip noma ku-Lateral Flow Assay Device.
Ulwelwesi lweNitrocellulose lusebenza njengomzimba ophethe ama-antibody asecaleni le-lateral flow assay device. Zisetshenziswa futhi ngenhloso yokuhlolwa kwezinqubo ezahlukahlukene nezinhlobo zama-antibodies okungukuthi i-monoclonals futhi lokhu kwenzeka njengomphumela wokuguqulwa kwe-enzymoimmounoassay.
Kulwelwesi lwe-nitrocellulose , ama-antibodies ahlala futhi ahlangane nezinhlayiya zezinto eziphilayo kungaba yi-latex noma izinhlayiya zegolide. Ama-antibodies awavunyelwe ukuhamba futhi ayavinjelwa kulwelwesi lwe-nitrocellulose ngenxa yezizathu ezahlukahlukene. Njengoba kunemigqa yokuhlola kanye nemigqa yokulawula ekuhlolweni kokugeleza kwe-lateral, ama-antibodies anamathele phakathi kwale migqa emibili. Uma ubheka umugqa wokuhlola, usetshenziselwa ukubopha iphrotheni yesampula kanti umugqa wokulawula une-antibodies ethize yohlobo esetshenziselwa ukuthola ama-antibodies kusampula. Esinye isizathu sokuthi ama-antibodies ahlala kulwelwesi lwe-nitrocellulose usayizi wembobo yefilimu engemuva. Ifilimu engemuva engenambobo yembobo yembobo ye-nitrocellulose ayivumeli ama-antibodies ukuthi asheleleke kunalokho aphathwa kahle futhi ngaleyo ndlela ahlale kulwelwesi. Lokhu kusiza futhi ekunciphiseni ukuguquguquka.
Ezinye izinto ezibalulekile ezenza ama-antibodies ahlale kulwelwesi lwe-nitrocellulose ukukhetha i-antibody diluent, indlela yokomisa, ukuhlushwa kwama-antibodies okubamba, kanye nesilinganiso sokusabalalisa ama-reagent. Ukomisa kahle kuyadingeka ukuqinisekisa ukuthi ama-antibody okubamba ayahlanganiswa kulwelwesi; noma kunjalo, ama-membrane angemuva athatha isikhathi esiningi ukoma uma kuqhathaniswa nama-membrane angasekelwanga. Ngaphandle kobunzima, ukukhathazeka komsebenzisi, ukulungiswa kwesampula, ukugcinwa, ukuphindaphindeka, kanye nokuchazwa kwemiphumela okulula ukuyisebenzisa, kufanele kuqinisekiswe njalo ukuthi ukutholakala okubucayi kwamapharamitha acacisiwe kuyashesha. Ezinye zalezi zindinganiso, njengokuchazwa kwemiphumela kanye nokuzwela, kubangelwa kakhulu ukungasebenzi kahle kwama-ejenti okuthola kanye nokugcinwa kwendawo ngamapharamitha endawo yokuhlola.
Enye indlela ewusizo ekwenzeni ama-antibody ahlale kulwelwesi lwe-nitrocellulose ukunamathiselwa kwama-antibody kulwelwesi lwe-Lateral Flow Assay. Futhi, ukungasebenzi okungahlangani kwama-antibody kulwelwesi lwe-nitrocellulose lwe-LFA kusikisela ngamandla amakhulu kakhulu wezingqimba ze-nitrocellulose ekubambeni amasosha omzimba kuwo. Njengoba kushiwo ngenhla ukuthi kwi-Lateral Flow Assay kunezinhlayiya ezizwelayo futhi ezibalulekile ekuhlolweni kwesampula. Ukuxhumanisa lokhu nezinhlayiya ze-colloidal zegolide, kufakazele ukuthi kwenza ama-antigen ahlale kulwelwesi lwe-cellulose. Kule nqubo, isinyathelo sokuqala ukukhishwa kwe-antibody okungenziwanga okudingekayo ebusweni be-nanoparticle yegolide colloidal. Lokhu kungenye yezinselele kakhulu ku-Lateral Flow Assay. Ngale njongo, izinhlayiya zokuhlangana zenziwe kalula ezenza ama-antibody, ama-molecule nezinye izinto zihlale kulwelwesi lwe-nitrocellulose.
Lokhu kunikeza nokunamathiselwa okulula nokugqokwa kwama-antibodies ebusweni kufaka amanye ama-analytic kanye nama-biomolecule. Ngaphandle kwalapho ubuhlalu be-latex ku-lateral flow assay nabo buyasiza ekwenzeni ama-antibody ahlale kulwelwesi lwe-nitrocellulose. Futhi, ukuhlangana okungahambisani nakho kungasiza ekwenzeni ama-antibody ahlale kulwelwesi lwe-nitrocelluse lovivinyo lokuhlolwa kwe-lateral flow. Noma lezi zinqubo nezimbangela zingenza ama-antibody ahlale kulwelwesi lwe-nitrocellulose ye-Lateral Flow Assay, athatha isikhathi futhi izinqubo ezinde kakhulu Hhayi lokhu kuphela, kepha kudinga imishini emikhulu nezinye izinto futhi ezenza kube matasa futhi kakhulu ebizayo. Futhi, ezinye izimbangela zidinga ukwenziwa okusebenzayo kwe-pH futhi lokhu kuyinto ebanzi, ngakho-ke, izindlela ezintsha nezinqubo zifakiwe kudivayisi yeLateral Flow Assay eholela ekusetshenzisweni kwezinye izindlela. Ama-antibodies ambalwa ahlukaniswe ngokukhethekile ku-nitrocellulose ngokumunca umzimba. Njengoba kunezimpande ezijulile ocwaningweni, ama-antibody nawo angahlala kulwelwesi lwe-nitrocellulose ngenxa yokuxhumana okuthile kwe-hydrophobic ne-dipolar phakathi kwazo zombili izizinda zamaprotheni kanye ne-nitrocellulose, okuholela ekulondolozweni kokuma kwama-antibodies.
Isiphetho
Ukuphetha nokufingqa lokhu ngokubheka incazelo engenhla, i-nitrocellulose iyingxenye ebalulekile yokuhlolwa kokugeleza kwe-lateral kanye nedivayisi esetshenziselwa ukuhlola. Esinye sezici ezibaluleke kakhulu nezibalulekile ezinquma i-Lateral Flow Assay kanye nokuzwela kwayo yi-Substrate Membrane noma i-Nitcocellulose Membrane . Imigqa yokuhlola nokulawula idwetshwa phezu kolwelwesi ukuqinisekisa ukubopha okuhle kanye nokubanjwa kwama-antibodies. Ukumuncwa okungaqondile emigqeni yokuhlola nokulawula kungathinta ukuzwela kwemiphumela, okuholela enanini lemiphumela engamanga. Njengoba i-nitrocellulose ingenye yezingxenye zokuhlolwa kokugeleza kwe-lateral lapho isampula idluliselwa khona. Kusukela kusampula, ama-antibodies kanye nezingxenye ze-antigenic zesampula kanye nedivayisi ziyahlangana bese zidlulela engxenyeni elandelayo. Ukusuka lapho, isampula igeleza iye kulwelwesi lwe-nitrocellulose oluthatha ama-antibodies nama-antigen adingekayo ekuhlolweni. Izakhiwo kanye nezizathu ezenza ukuhlala kwe-antigen kulwelwesi lwe-nitrocellulose kuchazwe ngemininingwane engenhla ngaleyo ndlela kuhlanganisa indaba yephepha.
Okubhekwayo
https://www.creative-diagnostics.com/Immunochromatography-guide.htm
https://fnkprddata.blob.core.windows.net/domestic/download/pdf/IBS_A_guide_to_lateral_flow_immunoassays.pdf
https://pubmed.ncbi.nlm.nih.gov/6350463/
https://www.bakerlab.org/wp-content/uploads/2015/12/Holstien_Anal_Bioanal_Chem_2015.pdf
https://www.nature.com/articles/s41598-021-87072-7
https://www.researchgate.net/topic/Nitrocellulose